Projects per year
Abstract
Detecting atmospheric bioaerosols in a quantitative way is highly desirable for public health and safety. This work demonstrates that surface-enhanced Raman spectroscopy (SERS) is a simple and rapid analytical technique for the detection of atmospheric bioaerosols, on a Klarite substrate. For both simulated and ambient bioaerosols, this detection assay results in an increase in the enhancement factor of the Raman signal. We report a strong SERS signal generated by bioaerosols containing living Escherichia coli deposited on Klarite. Furthermore, we demonstrate that SERS mapping can be used to estimate the percentage of airborne, living Escherichia coli. Moreover, Klarite provides differently distinct SERS spectra at different bacterial growth phases, indicating its potential to identify changes occurring in the bacterial envelope. Finally, we applied SERS for the rapid detection of Escherichia coli in ambient bioaerosols without using time-consuming and laborious culture processes. Our results represent rapid, culture-free and label-free detection of airborne bacteria in the real-world environment.
Original language | English |
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Pages (from-to) | 277-285 |
Number of pages | 9 |
Journal | Analyst |
Volume | 145 |
Issue number | 1 |
Early online date | 12 Nov 2019 |
DOIs | |
Publication status | Published - 7 Jan 2020 |
Funding
This material is based upon work jointly supported by the National Natural Science Foundation of China (No. 21976030 and 21677037), the Ministry of Science and Technology of the People’s Republic of China (2016YFE0112200 and 2016YFC0202700), the Natural Science Foundation of Shanghai (No. 19ZR1471200 and 17ZR1440200), and the China Postdoctoral Science Foundation funded project (No. KLH1829019). V. K. V. acknowledges support from the Royal Society through the University Research Fellowships and grants URF\R\191016, RGF\EA\180228 and PEF2E\100000.
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry
- Environmental Chemistry
- Spectroscopy
- Electrochemistry
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- 2 Finished
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Fellowship - Extended nonlinear meta-chiroptical effects
Valev, V. (PI)
1/10/19 → 30/09/24
Project: Research council
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Fellowship - Chirality in the 21st century: enantiomorphing chiral plasmonic meta/nano-materials
Valev, V. (PI)
1/12/17 → 30/09/24
Project: Research council