TY - GEN
T1 - Kinetics of mycobacterium M156 for chiral biotransformations
AU - Caridis, K. A.
AU - Stuckey, D. C.
AU - Leak, D. J.
PY - 1994/1/1
Y1 - 1994/1/1
N2 - In order to design an extractive biotransformation process to carry out a continuous biotransformation for the production of chiral epoxides, experiments have been carried out to investigate the epoxidation reaction of propene to 1,2-epoxypropane, and the biotransformation of allyl phenyl ether to phenyl glycidyl ether by Mycobacterium sp. strain M156 alkene monooxygenase, in a conventional aqueous system. The maximum epoxidation rate was determined as 41 nmol propylene oxide/mg dwt of cells.min and 33.2 nmol phenyl glycidyl ether/mg dwt.min. Kinetic studies revealed the propene affinity parameter, Ks, to be 1.59 μM, and the maximum reaction rate Umax = 30 nmol PO/mg dwt.min, while the allyl phenyl ether affinity parameter, Ks, was found equal to 1.2 mM and the maximum reaction rate Umax = 25 nmol PGE/mg dwt.min.
AB - In order to design an extractive biotransformation process to carry out a continuous biotransformation for the production of chiral epoxides, experiments have been carried out to investigate the epoxidation reaction of propene to 1,2-epoxypropane, and the biotransformation of allyl phenyl ether to phenyl glycidyl ether by Mycobacterium sp. strain M156 alkene monooxygenase, in a conventional aqueous system. The maximum epoxidation rate was determined as 41 nmol propylene oxide/mg dwt of cells.min and 33.2 nmol phenyl glycidyl ether/mg dwt.min. Kinetic studies revealed the propene affinity parameter, Ks, to be 1.59 μM, and the maximum reaction rate Umax = 30 nmol PO/mg dwt.min, while the allyl phenyl ether affinity parameter, Ks, was found equal to 1.2 mM and the maximum reaction rate Umax = 25 nmol PGE/mg dwt.min.
UR - http://www.scopus.com/inward/record.url?scp=0027986204&partnerID=8YFLogxK
M3 - Chapter in a published conference proceeding
AN - SCOPUS:0027986204
SN - 0852953348
T3 - Institution of Chemical Engineers Symposium Series
SP - 37
EP - 39
BT - Institution of Chemical Engineers Symposium Series
A2 - Anon, null
PB - The Institution of Chemical Engineers
T2 - Proceedings of the Conference on Applied Biocatalysis
Y2 - 4 July 1994 through 6 July 1994
ER -