Kinetics of mycobacterium M156 for chiral biotransformations

K. A. Caridis, D. C. Stuckey, D. J. Leak

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In order to design an extractive biotransformation process to carry out a continuous biotransformation for the production of chiral epoxides, experiments have been carried out to investigate the epoxidation reaction of propene to 1,2-epoxypropane, and the biotransformation of allyl phenyl ether to phenyl glycidyl ether by Mycobacterium sp. strain M156 alkene monooxygenase, in a conventional aqueous system. The maximum epoxidation rate was determined as 41 nmol propylene oxide/mg dwt of cells.min and 33.2 nmol phenyl glycidyl ether/mg dwt.min. Kinetic studies revealed the propene affinity parameter, Ks, to be 1.59 μM, and the maximum reaction rate Umax = 30 nmol PO/mg dwt.min, while the allyl phenyl ether affinity parameter, Ks, was found equal to 1.2 mM and the maximum reaction rate Umax = 25 nmol PGE/mg dwt.min.

Original languageEnglish
Title of host publicationInstitution of Chemical Engineers Symposium Series
Editors Anon
PublisherThe Institution of Chemical Engineers
Pages37-39
Number of pages3
ISBN (Print)0852953348
Publication statusPublished - 1 Jan 1994
EventProceedings of the Conference on Applied Biocatalysis - Brighton, UK
Duration: 4 Jul 19946 Jul 1994

Publication series

NameInstitution of Chemical Engineers Symposium Series
ISSN (Print)0307-0492

Conference

ConferenceProceedings of the Conference on Applied Biocatalysis
CityBrighton, UK
Period4/07/946/07/94

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Engineering(all)

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