Abstract
Miscanthus x giganteus is a source of platform chemicals and bioethanol through fermentation. Cinnamates in leaves and stems were analysed by LC-ESI-MS(n). Free phenols were extracted and separated chromatographically. More than 20 hydroxycinnamates were identified by UV and LC-ESI-MS(n). Comparative LC-MS studies on the leaf extract showed isomers of O-caffeoylquinic acid (3-CQA, 4-CQA and 5-CQA), O-feruloylquinic acid (3-FQA, 4-FQA and 5-FQA) and para-coumaroylquinic acid (3-pCoQA and 5-pCoQA). Excepting 3-pCoQA, all were also detected in stem. 5-CQA dominated in leaf: a mandelonitrile-caffeoylquinic acid dominated in stem. Three minor leaf components were distinguished by fragmentation patterns in a targetted MS2 experiment as dicaffeoylquinic acid isomers. Others (M(r) 516) were tentatively identified as hexosylcaffeoyl-quinates. Three positional isomers of O-caffeoylshikimic acid were minor components. p-Hydroxybenzaldehyde was also a major component in stem. This is the first report of the hydroxycinnamic acid profile of leaves and stems of M. x giganteus.
Original language | English |
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Pages (from-to) | 2376-2384 |
Number of pages | 9 |
Journal | Phytochemistry |
Volume | 72 |
Issue number | 18 |
DOIs | |
Publication status | Published - Dec 2011 |
Keywords
- LC-ESI-MS(n)
- high-value chemicals
- hydroxycinnamic esters
- miscanthus x giganteus leaf and stem tissue
- UV
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Electrospray Time-of-Flight Mass Spectrometer (Open-Access)
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