Isolation, culture, and characterisation of mouse embryonic oesophagus and intestine

J M Quinian, Wei-Yuan Yu, David Tosh

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The gastrointestinal tract of vertebrates is lined by epithelium that develops from the endodermal germ layer. The oesophagus and intestine form part of the gastrointestinal tract and studying the normal development of both tissues is difficult due to lack of suitable in vitro model systems. One of the criteria for a reliable culture model includes the ability to carry out real-time observations in vitro. The method we describe here is based on the isolation of embryonic oesophagus and intestine from 11.5-day-old embryos and culture on fibronectin-coated coverslips in basal Eagle's medium and 20% fetal bovine serum. This model permits real-time observations of both tissues and over a few days in culture, markers of differentiation appear. This culture system appears to recapitulate normal oesophagus and intestine development.
Original languageEnglish
Title of host publicationMouse Cell Culture: Methods and Protocols
EditorsA Ward, D Tosh
PublisherHumana Press
Pages81-90
Number of pages10
Edition633
ISBN (Print)9781588297723
DOIs
Publication statusPublished - 2010

Publication series

NameMethods in Molecular Biology
PublisherHumana Press

Fingerprint

esophagus
intestines
gastrointestinal system
mice
fibronectins
fetal bovine serum
eagles
embryo (animal)
epithelium
vertebrates
tissues
methodology

Cite this

Quinian, J. M., Yu, W-Y., & Tosh, D. (2010). Isolation, culture, and characterisation of mouse embryonic oesophagus and intestine. In A. Ward, & D. Tosh (Eds.), Mouse Cell Culture: Methods and Protocols (633 ed., pp. 81-90). (Methods in Molecular Biology). Humana Press. https://doi.org/10.1007/978-1-59745-019-5_6

Isolation, culture, and characterisation of mouse embryonic oesophagus and intestine. / Quinian, J M; Yu, Wei-Yuan; Tosh, David.

Mouse Cell Culture: Methods and Protocols. ed. / A Ward; D Tosh. 633. ed. Humana Press, 2010. p. 81-90 (Methods in Molecular Biology).

Research output: Chapter in Book/Report/Conference proceedingChapter

Quinian, JM, Yu, W-Y & Tosh, D 2010, Isolation, culture, and characterisation of mouse embryonic oesophagus and intestine. in A Ward & D Tosh (eds), Mouse Cell Culture: Methods and Protocols. 633 edn, Methods in Molecular Biology, Humana Press, pp. 81-90. https://doi.org/10.1007/978-1-59745-019-5_6
Quinian JM, Yu W-Y, Tosh D. Isolation, culture, and characterisation of mouse embryonic oesophagus and intestine. In Ward A, Tosh D, editors, Mouse Cell Culture: Methods and Protocols. 633 ed. Humana Press. 2010. p. 81-90. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-59745-019-5_6
Quinian, J M ; Yu, Wei-Yuan ; Tosh, David. / Isolation, culture, and characterisation of mouse embryonic oesophagus and intestine. Mouse Cell Culture: Methods and Protocols. editor / A Ward ; D Tosh. 633. ed. Humana Press, 2010. pp. 81-90 (Methods in Molecular Biology).
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