Isolation and functional characterization of an allatotropin receptor from Manduca sexta

F M Horodyski, H Verlinden, N Filkin, H P Vandersmissen, Christophe Fleury, Stuart E Reynolds, Z P Kai, J V Broeck

Research output: Contribution to journalArticle

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Abstract

Manduca sexta allatotropin (Manse-AT) is a multifunctional neuropeptide whose actions include the stimulation of juvenile hormone biosynthesis, myotropic stimulation, cardioacceleratory functions, and inhibition of active ion transport. Manse-AT is a member of a structurally related peptide family that is widely found in insects and also in other invertebrates. Its precise role depends on the insect species and developmental stage. In some lepidopteran insects including M. sexta, structurally-related AT-like (An) peptides can be derived from alternatively spliced mRNAs transcribed from the AT gene. We have isolated a cDNA for an AT receptor (AIR) from M. sexta by a PCR-based approach using the sequence of the ATR from Bombyx mori. The sequence of the M. sexta ATR is similar to several G protein-coupled receptors from other insect species and to the mammalian orexin receptor. We demonstrate that the M. sexta ATR expressed in vertebrate cell lines is activated in a dose-responsive manner by Manse-AT and each Manse-An peptide in the rank order ATL-I > An-II > ATL-III > AT, and functional analysis in multiple cell lines suggest that the receptor is coupled through elevated levels of Ca(2+) and cAMP. In feeding larvae, Manse-ATR mRNA is present at highest levels in the Malpighian tubules, followed by the midgut, hindgut, testes, and corpora allata, consistent with its action on multiple target tissues. In the adult corpora cardiaca corpora allata complex, Manse-AIR mRNA is present at relatively low levels in both sexes.
LanguageEnglish
Pages804-814
Number of pages11
JournalInsect Biochemistry and Molecular Biology
Volume41
Issue number10
DOIs
StatusPublished - Oct 2011

Fingerprint

allatotropin
Manduca
Manduca sexta
Corpora Allata
Messenger RNA
Peptides
receptors
Insects
Orexin Receptors
Cells
Juvenile Hormones
Functional analysis
Biosynthesis
insects
G-Protein-Coupled Receptors
corpora allata
Neuropeptides
peptides
Complementary DNA
Genes

Keywords

  • G protein-coupled receptors
  • juvenile hormone
  • neuropeptides
  • manduca sexta
  • allatotropin

Cite this

Horodyski, F. M., Verlinden, H., Filkin, N., Vandersmissen, H. P., Fleury, C., Reynolds, S. E., ... Broeck, J. V. (2011). Isolation and functional characterization of an allatotropin receptor from Manduca sexta. Insect Biochemistry and Molecular Biology, 41(10), 804-814. https://doi.org/10.1016/j.ibmb.2011.06.002

Isolation and functional characterization of an allatotropin receptor from Manduca sexta. / Horodyski, F M; Verlinden, H; Filkin, N; Vandersmissen, H P; Fleury, Christophe; Reynolds, Stuart E; Kai, Z P; Broeck, J V.

In: Insect Biochemistry and Molecular Biology, Vol. 41, No. 10, 10.2011, p. 804-814.

Research output: Contribution to journalArticle

Horodyski, FM, Verlinden, H, Filkin, N, Vandersmissen, HP, Fleury, C, Reynolds, SE, Kai, ZP & Broeck, JV 2011, 'Isolation and functional characterization of an allatotropin receptor from Manduca sexta', Insect Biochemistry and Molecular Biology, vol. 41, no. 10, pp. 804-814. https://doi.org/10.1016/j.ibmb.2011.06.002
Horodyski, F M ; Verlinden, H ; Filkin, N ; Vandersmissen, H P ; Fleury, Christophe ; Reynolds, Stuart E ; Kai, Z P ; Broeck, J V. / Isolation and functional characterization of an allatotropin receptor from Manduca sexta. In: Insect Biochemistry and Molecular Biology. 2011 ; Vol. 41, No. 10. pp. 804-814.
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