Isolation and culture of embryonic pancreas and liver

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Citations (Scopus)

Abstract

Culturing embryonic tissue in an in vitro setting offers the unique ability to manipulate the external medium and therefore to investigate the pathways involved in regulating normal organogenesis as well as providing models for developmental disorders. Here we describe a system for the in vitro culture of the dorsal pancreatic buds and liver buds from mouse embryos. The tissues are dissected from day 9.0 or 11.5 mouse embryos. The tissues are placed on fibronectin-coated coverslips in serum-containing medium and allowed to attach. Over the next few days, the buds grow as flattened structures which are thin enough to allow the use of wholemount immunostaining methods.
Original languageEnglish
Title of host publicationMouse Cell Culture: Methods and Protocols
EditorsA Ward, D Tosh
PublisherHumana Press
Pages91-99
Number of pages9
Edition633
ISBN (Print)9781588297723
DOIs
Publication statusPublished - 2010

Publication series

NameMethods in Molecular Biology
PublisherHumana Press

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  • Projects

    BETA CELLS IN THE LIVER

    Tosh, D. & Slack, J.

    Medical Research Council

    1/01/0631/12/08

    Project: Research council

  • Cite this

    Burke, Z. D., Li, W-C., Slack, J., & Tosh, D. (2010). Isolation and culture of embryonic pancreas and liver. In A. Ward, & D. Tosh (Eds.), Mouse Cell Culture: Methods and Protocols (633 ed., pp. 91-99). (Methods in Molecular Biology). Humana Press. https://doi.org/10.1007/978-1-59745-019-5_7