Isolation and culture of adult mouse hepatocytes

Wan-Chun Li, Kate L Ralphs, David Tosh

Research output: Chapter in Book/Report/Conference proceedingChapter

118 Citations (Scopus)

Abstract

The liver performs a multitude of functions including the regulation of carbohydrate, fat, and protein metabolism, the detoxification of endo- and xenobiotics, and the synthesis and secretion of plasma proteins and bile. Isolated hepatocytes constitute a useful technique for studying liver function in an in vitro setting. Here we describe a method for the isolation of hepatocytes from adult mouse liver. The principle of the method is the two-step collagenase perfusion technique which involves sequential perfusion of the liver with ethylenediaminetetraacetic acid and collagenase. Following isolation, the cells can either be used for short-term studies or, alternatively, maintained in culture for prolonged periods to study long-term changes in gene expression. The protocol for mouse hepatocyte isolation may be applied to both normal and transgenic mice.
Original languageEnglish
Title of host publicationMouse Cell Culture: Methods and Protocols
EditorsA Ward, D Tosh
PublisherHumana Press
Pages185-196
Number of pages12
Edition633
ISBN (Print)9781588297723
DOIs
Publication statusPublished - 2010

Publication series

NameMethods in Molecular Biology
PublisherHumana Press

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Hepatocytes
Liver
Collagenases
Perfusion
Xenobiotics
Bile
Edetic Acid
Transgenic Mice
Blood Proteins
Fats
Carbohydrates
Gene Expression
Proteins

Cite this

Li, W-C., Ralphs, K. L., & Tosh, D. (2010). Isolation and culture of adult mouse hepatocytes. In A. Ward, & D. Tosh (Eds.), Mouse Cell Culture: Methods and Protocols (633 ed., pp. 185-196). (Methods in Molecular Biology). Humana Press. https://doi.org/10.1007/978-1-59745-019-5_13

Isolation and culture of adult mouse hepatocytes. / Li, Wan-Chun; Ralphs, Kate L; Tosh, David.

Mouse Cell Culture: Methods and Protocols. ed. / A Ward; D Tosh. 633. ed. Humana Press, 2010. p. 185-196 (Methods in Molecular Biology).

Research output: Chapter in Book/Report/Conference proceedingChapter

Li, W-C, Ralphs, KL & Tosh, D 2010, Isolation and culture of adult mouse hepatocytes. in A Ward & D Tosh (eds), Mouse Cell Culture: Methods and Protocols. 633 edn, Methods in Molecular Biology, Humana Press, pp. 185-196. https://doi.org/10.1007/978-1-59745-019-5_13
Li W-C, Ralphs KL, Tosh D. Isolation and culture of adult mouse hepatocytes. In Ward A, Tosh D, editors, Mouse Cell Culture: Methods and Protocols. 633 ed. Humana Press. 2010. p. 185-196. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-59745-019-5_13
Li, Wan-Chun ; Ralphs, Kate L ; Tosh, David. / Isolation and culture of adult mouse hepatocytes. Mouse Cell Culture: Methods and Protocols. editor / A Ward ; D Tosh. 633. ed. Humana Press, 2010. pp. 185-196 (Methods in Molecular Biology).
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