Isolation and culture of adult mouse hepatocytes

Wan-Chun Li, Kate L Ralphs, David Tosh

Research output: Chapter in Book/Report/Conference proceedingChapter

154 Citations (Scopus)

Abstract

The liver performs a multitude of functions including the regulation of carbohydrate, fat, and protein metabolism, the detoxification of endo- and xenobiotics, and the synthesis and secretion of plasma proteins and bile. Isolated hepatocytes constitute a useful technique for studying liver function in an in vitro setting. Here we describe a method for the isolation of hepatocytes from adult mouse liver. The principle of the method is the two-step collagenase perfusion technique which involves sequential perfusion of the liver with ethylenediaminetetraacetic acid and collagenase. Following isolation, the cells can either be used for short-term studies or, alternatively, maintained in culture for prolonged periods to study long-term changes in gene expression. The protocol for mouse hepatocyte isolation may be applied to both normal and transgenic mice.
Original languageEnglish
Title of host publicationMouse Cell Culture: Methods and Protocols
EditorsA Ward, D Tosh
PublisherHumana Press
Pages185-196
Number of pages12
Edition633
ISBN (Print)9781588297723
DOIs
Publication statusPublished - 2010

Publication series

NameMethods in Molecular Biology
PublisherHumana Press

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