Isocratic separation of monosaccharides using immobilized Concanavalin A

F Santori, J Hubble

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The successful fractionation of a mixt. of monosaccharides using an immobilized lectin column operating under isocratic conditions is described. The key factors for effective sepn. were found to be sample size, feed velocity and column length. Under optimal conditions it was possible to obtain complete resoln. of a mixt. of L-arabinose, D-fructose and D-mannose in less than 40 min. [on SciFinder (R)]
Original languageEnglish
Pages (from-to)123-126
Number of pages4
JournalJournal of Chromatography A
Volume1003
Issue number1-2
Publication statusPublished - 2003

Keywords

  • HPLC
  • Affinity chromatography
  • ANST (Analytical study) (isocratic HPLC sepn. of monosaccharides using immobilized Con A as affinity stationary phase)
  • Immobilization (isocratic HPLC sepn. of monosaccharides using immobilized Con A as affinity stationary phase)
  • HPLC stationary phases
  • affinity chromatog isocratic sepn monosaccharide immobilized concanavalin
  • ANT (Analyte)
  • Monosaccharides Role

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