Iontophoretic transdermal sampling of iohexol as a noninvasive tool to assess glomerular filtration rate

Asma Djabri, William van’t Hoff, Penelope Brock, Ian C.K. Wong, Richard Guy, Begona Delgado-Charro

Research output: Contribution to journalArticle

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110 Downloads (Pure)

Abstract

Purpose

To explore the potential of non-invasive reverse iontophoresis transdermal extraction of iohexol as a marker of glomerular filtration rate.

Methods

A series of in vitro experiments were undertaken to establish the feasibility of iohexol reverse iontophoresis and to determine the optimal conditions for the approach. Subsequently, a pilot study in paediatric patients was performed to provide proof-of-concept.

Results

The iontophoretic extraction fluxes of iohexol in vitro were proportional to the marker subdermal concentration and the reverse iontophoretic technique was able to track changes dynamically in simulated pharmacokinetic profiles. Reverse iontophoresis sampling was well tolerated by the four paediatric participants. The deduced values of the iohexol terminal elimination rate constant from transdermal reverse iontophoresis sampling agreed with those estimated by conventional blood sampling.

Conclusions

Reverse iontophoretic transdermal extraction fluxes mirrored the subdermal concentration profiles of iohexol, a relatively large neutral marker of glomerular filtration both in vitro and in vivo. The efficiency of extraction in vivo was well predicted by the in vitro model used.
Original languageEnglish
Pages (from-to)590-603
Number of pages14
JournalPharmaceutical Research
Volume32
Issue number2
Early online date5 Sep 2014
DOIs
Publication statusPublished - 1 Feb 2015

Fingerprint

Iohexol
Iontophoresis
Glomerular Filtration Rate
Sampling
Pediatrics
Fluxes
Pharmacokinetics
Rate constants
Blood
In Vitro Techniques
Experiments

Keywords

  • GFR
  • iohexol
  • iontophoresis
  • non-invasive monitoring
  • transdermal sampling

Cite this

Iontophoretic transdermal sampling of iohexol as a noninvasive tool to assess glomerular filtration rate. / Djabri, Asma; van’t Hoff, William; Brock, Penelope; Wong, Ian C.K.; Guy, Richard; Delgado-Charro, Begona.

In: Pharmaceutical Research, Vol. 32, No. 2, 01.02.2015, p. 590-603.

Research output: Contribution to journalArticle

Djabri, Asma ; van’t Hoff, William ; Brock, Penelope ; Wong, Ian C.K. ; Guy, Richard ; Delgado-Charro, Begona. / Iontophoretic transdermal sampling of iohexol as a noninvasive tool to assess glomerular filtration rate. In: Pharmaceutical Research. 2015 ; Vol. 32, No. 2. pp. 590-603.
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AB - PurposeTo explore the potential of non-invasive reverse iontophoresis transdermal extraction of iohexol as a marker of glomerular filtration rate.MethodsA series of in vitro experiments were undertaken to establish the feasibility of iohexol reverse iontophoresis and to determine the optimal conditions for the approach. Subsequently, a pilot study in paediatric patients was performed to provide proof-of-concept.ResultsThe iontophoretic extraction fluxes of iohexol in vitro were proportional to the marker subdermal concentration and the reverse iontophoretic technique was able to track changes dynamically in simulated pharmacokinetic profiles. Reverse iontophoresis sampling was well tolerated by the four paediatric participants. The deduced values of the iohexol terminal elimination rate constant from transdermal reverse iontophoresis sampling agreed with those estimated by conventional blood sampling.ConclusionsReverse iontophoretic transdermal extraction fluxes mirrored the subdermal concentration profiles of iohexol, a relatively large neutral marker of glomerular filtration both in vitro and in vivo. The efficiency of extraction in vivo was well predicted by the in vitro model used.

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