TY - JOUR
T1 - Iontophoretic permselectivity of mammalian skin
T2 - characterization of hairless mouse and porcine membrane models
AU - Luzardo-Alvarez, Asteria
AU - Rodríguez-Fernández, Monica
AU - Blanco-Méndez, José
AU - Guy, Richard H.
AU - Delgado-Charro, M. Begoña
PY - 1998/7
Y1 - 1998/7
N2 - Purpose. To evaluate the transport number of Na+, and the isoelectric point, of two skin membranes frequently used for iontophoretic in vitro research. Methods. Na+ transport numbers were determined by the Hittorf method or by the measurement of membrane potential. The skin isoelectric point was deduced from the electroosmosis of mannitol (a polar non-electrolyte) as a function of pH. Results. The Na+ transport number across porcine skin, like that for hairless mouse, indicated a modest cation permselectivity. Consistent with this observation, the isoelectric points of porcine and hairless mouse skin were determined to be in the ranges of 3.5-3.75 and 4.5-4.6, respectively. That is, at physiological pH, both of these model membranes supports a net negative charge. Conclusions. The permselective properties of porcine and hairless mouse skin are similar (but with the porcine membrane having apparently fewer basic or more weakly-acidic groups than that of the mouse) and consistent with the characteristics, which have been deduced elsewhere, of human skin.
AB - Purpose. To evaluate the transport number of Na+, and the isoelectric point, of two skin membranes frequently used for iontophoretic in vitro research. Methods. Na+ transport numbers were determined by the Hittorf method or by the measurement of membrane potential. The skin isoelectric point was deduced from the electroosmosis of mannitol (a polar non-electrolyte) as a function of pH. Results. The Na+ transport number across porcine skin, like that for hairless mouse, indicated a modest cation permselectivity. Consistent with this observation, the isoelectric points of porcine and hairless mouse skin were determined to be in the ranges of 3.5-3.75 and 4.5-4.6, respectively. That is, at physiological pH, both of these model membranes supports a net negative charge. Conclusions. The permselective properties of porcine and hairless mouse skin are similar (but with the porcine membrane having apparently fewer basic or more weakly-acidic groups than that of the mouse) and consistent with the characteristics, which have been deduced elsewhere, of human skin.
KW - Electroosmosis
KW - Iontophoresis
KW - Mannitol
KW - Permselectivity
KW - Skin isoelectric point
KW - Transdermal delivery
UR - http://www.scopus.com/inward/record.url?scp=0031822972&partnerID=8YFLogxK
UR - http://dx.doi.org/10.1023/A:1011909623019
U2 - 10.1023/A:1011909623019
DO - 10.1023/A:1011909623019
M3 - Article
C2 - 9688048
AN - SCOPUS:0031822972
SN - 0724-8741
VL - 15
SP - 984
EP - 987
JO - Pharmaceutical Research
JF - Pharmaceutical Research
IS - 7
ER -