Involvement of adenosine in ethanol-induced changes in G-protein expression in NG108-15 cells?

Robert J Williams, M A Veale, E Kelly

Research output: Contribution to journalArticle

Abstract

Previous work has suggested that adenosine may be involved in ethanol-induced heterologous desensitization of adenylate cyclase in NG108-15 cells. It was proposed that chronic ethanol causes adenosine to accumulate extracellularly, activating adenosine A2 receptors and so leading to a reduction in Gs alpha mRNA and Gs alpha protein (Nagy et al., 1989). In this study we further investigated the effect of chronic ethanol on G-protein expression in NG108-15 cells. Pretreatment of NG108-15 cells with ethanol (200 mM, 48h) reduced membrane levels of Gs alpha and Gi alpha but increased Go alpha expression. The effects of ethanol on alpha-subunit expression were not reversed by adenosine deaminase and could not be mimicked by the adenosine agonist 5'- (n-ethyl)-carboxamidoadenosine (NECA). Chronic ethanol pretreatment did not appear to reduce the levels of Gs alpha or Gi alpha 2 mRNA. This same ethanol pretreatment reduced cell proliferation and increased differentiation without altering cell viability. Adenosine deaminase did not reverse any of these effects. These results indicate that ethanol differentially regulates G-protein alpha-subunit expression and induces morphological alterations in these cells independently of extracellular adenosine.
Original languageEnglish
Pages (from-to)431-435
Number of pages5
JournalAlcohol and Alcoholism: Supplement
Volume2
Publication statusPublished - 1993

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GTP-Binding Proteins
Adenosine
Ethanol
Adenosine Deaminase
Adenosine A2 Receptors
Gs GTP-Binding Protein alpha Subunits
GTP-Binding Protein alpha Subunits
Messenger RNA
Adenylyl Cyclases
Cell Survival
Cell Proliferation
Membranes
Proteins

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Involvement of adenosine in ethanol-induced changes in G-protein expression in NG108-15 cells? / Williams, Robert J; Veale, M A; Kelly, E.

In: Alcohol and Alcoholism: Supplement, Vol. 2, 1993, p. 431-435.

Research output: Contribution to journalArticle

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AB - Previous work has suggested that adenosine may be involved in ethanol-induced heterologous desensitization of adenylate cyclase in NG108-15 cells. It was proposed that chronic ethanol causes adenosine to accumulate extracellularly, activating adenosine A2 receptors and so leading to a reduction in Gs alpha mRNA and Gs alpha protein (Nagy et al., 1989). In this study we further investigated the effect of chronic ethanol on G-protein expression in NG108-15 cells. Pretreatment of NG108-15 cells with ethanol (200 mM, 48h) reduced membrane levels of Gs alpha and Gi alpha but increased Go alpha expression. The effects of ethanol on alpha-subunit expression were not reversed by adenosine deaminase and could not be mimicked by the adenosine agonist 5'- (n-ethyl)-carboxamidoadenosine (NECA). Chronic ethanol pretreatment did not appear to reduce the levels of Gs alpha or Gi alpha 2 mRNA. This same ethanol pretreatment reduced cell proliferation and increased differentiation without altering cell viability. Adenosine deaminase did not reverse any of these effects. These results indicate that ethanol differentially regulates G-protein alpha-subunit expression and induces morphological alterations in these cells independently of extracellular adenosine.

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