Investigating the effect of tobramycin dry powder inhaler on the eradication of Pseudomonas aeruginosa biofilms

Research output: Contribution to journalMeeting abstract

Abstract

Biofilms are sessile communities of microorganisms embedded within a self-generated extracellular polymeric matrix, which consists of polysaccharides, DNA, and/or proteins. Such biofilms are found for instance in adults with cystic fibrosis, with pulmonary infections with the Gram-negative bacterium Pseudomonas aeruginosa being particularly common and responsible for a high mortality rate among CF patients. This infection in CF patients is commonly managed with antibiotic dry powder inhalers, one of which is the aminoglycoside tobramycin. The activity of tobramycin has been well characterized in vitro, but current models that have been used are not very representative for lung infections, and better models would provide a significant advantage as these could be used, for instance, to improve the formulation of dry powder inhalers.
For instance, one question that has not been addressed with current models is whether the size of drug particles emitted from a dry powder inhaler influences the efficacy of the anti-biofilm activity of the antibiotic. In this project, we utilized the Next Generation Impactor (NGI), which is a pharmaceutical instrument used to separate particles into size fractions. We used the NGI to separate tobramycin particles into different sizes and tested the influence of these particles on eradication of P. aeruginosa biofilms, which were grown using as colony biofilms that closely mimics conditions in the lung where biofilms are grown on a substrate-air interface. Preliminary evidence indicated smaller tobramycin particles are better in eradication of P. aeruginosa biofilms as compared to larger particles. Our results may represent a step towards improving the formulation of tobramycin dry powder inhalers to be effective in eradicating P. aeruginosa biofilms.
LanguageEnglish
Number of pages1
JournalAccess Microbiology
Volume1
Issue number1A
DOIs
StatusPublished - 8 Apr 2019

Keywords

  • P. aeruginosa biofilms, tobramycin dry powder inhaler, NGI, CF lung infections

Cite this

@article{30c71f58e32d4aa1866fbac1c086d327,
title = "Investigating the effect of tobramycin dry powder inhaler on the eradication of Pseudomonas aeruginosa biofilms",
abstract = "Biofilms are sessile communities of microorganisms embedded within a self-generated extracellular polymeric matrix, which consists of polysaccharides, DNA, and/or proteins. Such biofilms are found for instance in adults with cystic fibrosis, with pulmonary infections with the Gram-negative bacterium Pseudomonas aeruginosa being particularly common and responsible for a high mortality rate among CF patients. This infection in CF patients is commonly managed with antibiotic dry powder inhalers, one of which is the aminoglycoside tobramycin. The activity of tobramycin has been well characterized in vitro, but current models that have been used are not very representative for lung infections, and better models would provide a significant advantage as these could be used, for instance, to improve the formulation of dry powder inhalers. For instance, one question that has not been addressed with current models is whether the size of drug particles emitted from a dry powder inhaler influences the efficacy of the anti-biofilm activity of the antibiotic. In this project, we utilized the Next Generation Impactor (NGI), which is a pharmaceutical instrument used to separate particles into size fractions. We used the NGI to separate tobramycin particles into different sizes and tested the influence of these particles on eradication of P. aeruginosa biofilms, which were grown using as colony biofilms that closely mimics conditions in the lung where biofilms are grown on a substrate-air interface. Preliminary evidence indicated smaller tobramycin particles are better in eradication of P. aeruginosa biofilms as compared to larger particles. Our results may represent a step towards improving the formulation of tobramycin dry powder inhalers to be effective in eradicating P. aeruginosa biofilms.",
keywords = "P. aeruginosa biofilms, tobramycin dry powder inhaler, NGI, CF lung infections",
author = "Reham Aljalamdeh and Robert Price and Matthew Jones and Albert Bolhuis",
year = "2019",
month = "4",
day = "8",
doi = "10.1099/acmi.ac2019.po0039",
language = "English",
volume = "1",
journal = "Access Microbiology",
issn = "2516-8290",
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T1 - Investigating the effect of tobramycin dry powder inhaler on the eradication of Pseudomonas aeruginosa biofilms

AU - Aljalamdeh, Reham

AU - Price, Robert

AU - Jones, Matthew

AU - Bolhuis, Albert

PY - 2019/4/8

Y1 - 2019/4/8

N2 - Biofilms are sessile communities of microorganisms embedded within a self-generated extracellular polymeric matrix, which consists of polysaccharides, DNA, and/or proteins. Such biofilms are found for instance in adults with cystic fibrosis, with pulmonary infections with the Gram-negative bacterium Pseudomonas aeruginosa being particularly common and responsible for a high mortality rate among CF patients. This infection in CF patients is commonly managed with antibiotic dry powder inhalers, one of which is the aminoglycoside tobramycin. The activity of tobramycin has been well characterized in vitro, but current models that have been used are not very representative for lung infections, and better models would provide a significant advantage as these could be used, for instance, to improve the formulation of dry powder inhalers. For instance, one question that has not been addressed with current models is whether the size of drug particles emitted from a dry powder inhaler influences the efficacy of the anti-biofilm activity of the antibiotic. In this project, we utilized the Next Generation Impactor (NGI), which is a pharmaceutical instrument used to separate particles into size fractions. We used the NGI to separate tobramycin particles into different sizes and tested the influence of these particles on eradication of P. aeruginosa biofilms, which were grown using as colony biofilms that closely mimics conditions in the lung where biofilms are grown on a substrate-air interface. Preliminary evidence indicated smaller tobramycin particles are better in eradication of P. aeruginosa biofilms as compared to larger particles. Our results may represent a step towards improving the formulation of tobramycin dry powder inhalers to be effective in eradicating P. aeruginosa biofilms.

AB - Biofilms are sessile communities of microorganisms embedded within a self-generated extracellular polymeric matrix, which consists of polysaccharides, DNA, and/or proteins. Such biofilms are found for instance in adults with cystic fibrosis, with pulmonary infections with the Gram-negative bacterium Pseudomonas aeruginosa being particularly common and responsible for a high mortality rate among CF patients. This infection in CF patients is commonly managed with antibiotic dry powder inhalers, one of which is the aminoglycoside tobramycin. The activity of tobramycin has been well characterized in vitro, but current models that have been used are not very representative for lung infections, and better models would provide a significant advantage as these could be used, for instance, to improve the formulation of dry powder inhalers. For instance, one question that has not been addressed with current models is whether the size of drug particles emitted from a dry powder inhaler influences the efficacy of the anti-biofilm activity of the antibiotic. In this project, we utilized the Next Generation Impactor (NGI), which is a pharmaceutical instrument used to separate particles into size fractions. We used the NGI to separate tobramycin particles into different sizes and tested the influence of these particles on eradication of P. aeruginosa biofilms, which were grown using as colony biofilms that closely mimics conditions in the lung where biofilms are grown on a substrate-air interface. Preliminary evidence indicated smaller tobramycin particles are better in eradication of P. aeruginosa biofilms as compared to larger particles. Our results may represent a step towards improving the formulation of tobramycin dry powder inhalers to be effective in eradicating P. aeruginosa biofilms.

KW - P. aeruginosa biofilms, tobramycin dry powder inhaler, NGI, CF lung infections

U2 - 10.1099/acmi.ac2019.po0039

DO - 10.1099/acmi.ac2019.po0039

M3 - Meeting abstract

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