Inter-laboratory validation of procedures for measuring 8-oxo-7,8-dihydroguanine/8-oxo-7,8-dihydro-2 '-deoxyguanosine in DNA

A R Collins; C Gedik; S Wood; A White; J Dubois; P Duez; J F Rees; R Legall; L Degand; S Loft; A Jensen; H Poulsen; A Weimann; B R Jensen; J Cadet; T Douki; J L Ravanat; H Faure; M Tripier; I Morel; O Sergent; P Cillard; B Morin; B Epe; N Phoa; A Hartwig; A Pelzer; P Dolara; C Casalini; F Guglielmi; C Luceri; H Kasai; R Kido; R Olinski; K Bialkowski; Z Durackova; L Hlincikova; P Korytar; M Dusinska; C Mislanova; J Vina; A Lloret; L Moller; T Hofer; E Gremaud; L Fay; R Stadler; J Eakins; F Pognan; J O'Brien; R Elliott; S Astley; A Bailley; K Herbert; D Chauhan; F Kelly; C Dunster; J Lunec; I Podmore; P Patel; S Johnson; M Evans; R Tyrrell; M Gordon; C Wild; L Hardie; E Smith; [No Value] Escodd

doi:10.1080/10715760290019246

Inter-laboratory validation of procedures for measuring 8-oxo-7,8-dihydroguanine/8-oxo-7,8-dihydro-2 '-deoxyguanosine in DNA

A R Collins, C Gedik, S Wood, A White, J Dubois, P Duez, J F Rees, R Legall, L Degand, S Loft, A Jensen, H Poulsen, A Weimann, B R Jensen, J Cadet, T Douki, J L Ravanat, H Faure, M Tripier, I MorelO Sergent, P Cillard, B Morin, B Epe, N Phoa, A Hartwig, A Pelzer, P Dolara, C Casalini, F Guglielmi, C Luceri, H Kasai, R Kido, R Olinski, K Bialkowski, Z Durackova, L Hlincikova, P Korytar, M Dusinska, C Mislanova, J Vina, A Lloret, L Moller, T Hofer, E Gremaud, L Fay, R Stadler, J Eakins, F Pognan, J O'Brien, R Elliott, S Astley, A Bailley, K Herbert, D Chauhan, F Kelly, C Dunster, J Lunec, I Podmore, P Patel, S Johnson, M Evans, R Tyrrell, M Gordon, C Wild, L Hardie, E Smith, [No Value] Escodd

Department of Life Sciences

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Abstract

The aim of ESCODD, a European Commission funded Concerted Action, is to improve the precision and accuracy of methods for measuring 8-oxo-7,8-dihydroguanine (8-oxoGua) or the nucleoside (8-oxodG). On two occasions, participating laboratories received samples of different concentrations of 8-oxodG for analysis. About half the results returned (for 8-oxodG) were within 20% of the median values. Coefficients of variation (for three identical samples) were commonly around 10%. A sample of calf thymus DNA was sent, dry, to all laboratories. Analysis of 8-oxoGua/8-oxodG in this sample was a test of hydrolysis methods. Almost half the reported results were within 20% of the median value, and half obtained a CV of less than 10%. In order to test sensitivity, as well as precision, DNA was treated with photosensitiser and light to introduce increasing amounts of 8-oxoGua and samples were sent to members. Median values calculated from all returned results were 45.6 (untreated), 53.9, 60.4 and 65.6 8-oxoGua/10(6) Gua; only seven laboratories detected the increase over the whole range, while all but one detected a dose response over two concentration intervals. Results in this trial reflect a continuing improvement in precision and accuracy. The next challenge will be the analysis of 8-oxodG in DNA isolated from cells or tissue, where the concentration is much lower than in calf thymus DNA.

Original language	English
Pages (from-to)	239-245
Number of pages	7
Journal	Free Radical Research
Volume	36
Issue number	3
DOIs	https://doi.org/10.1080/10715760290019246
Publication status	Published - 2002

Bibliographical note

ID number: ISI:000174725300001

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10.1080/10715760290019246

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Collins, A. R., Gedik, C., Wood, S., White, A., Dubois, J., Duez, P., Rees, J. F., Legall, R., Degand, L., Loft, S., Jensen, A., Poulsen, H., Weimann, A., Jensen, B. R., Cadet, J., Douki, T., Ravanat, J. L., Faure, H., Tripier, M., ... Escodd, N. V. (2002). Inter-laboratory validation of procedures for measuring 8-oxo-7,8-dihydroguanine/8-oxo-7,8-dihydro-2 '-deoxyguanosine in DNA. Free Radical Research, 36(3), 239-245. https://doi.org/10.1080/10715760290019246

Collins, AR, Gedik, C, Wood, S, White, A, Dubois, J, Duez, P, Rees, JF, Legall, R, Degand, L, Loft, S, Jensen, A, Poulsen, H, Weimann, A, Jensen, BR, Cadet, J, Douki, T, Ravanat, JL, Faure, H, Tripier, M, Morel, I, Sergent, O, Cillard, P, Morin, B, Epe, B, Phoa, N, Hartwig, A, Pelzer, A, Dolara, P, Casalini, C, Guglielmi, F, Luceri, C, Kasai, H, Kido, R, Olinski, R, Bialkowski, K, Durackova, Z, Hlincikova, L, Korytar, P, Dusinska, M, Mislanova, C, Vina, J, Lloret, A, Moller, L, Hofer, T, Gremaud, E, Fay, L, Stadler, R, Eakins, J, Pognan, F, O'Brien, J, Elliott, R, Astley, S, Bailley, A, Herbert, K, Chauhan, D, Kelly, F, Dunster, C, Lunec, J, Podmore, I, Patel, P, Johnson, S, Evans, M, Tyrrell, R, Gordon, M, Wild, C, Hardie, L, Smith, E & Escodd, NV 2002, 'Inter-laboratory validation of procedures for measuring 8-oxo-7,8-dihydroguanine/8-oxo-7,8-dihydro-2 '-deoxyguanosine in DNA', Free Radical Research, vol. 36, no. 3, pp. 239-245. https://doi.org/10.1080/10715760290019246

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title = "Inter-laboratory validation of procedures for measuring 8-oxo-7,8-dihydroguanine/8-oxo-7,8-dihydro-2 '-deoxyguanosine in DNA",

abstract = "The aim of ESCODD, a European Commission funded Concerted Action, is to improve the precision and accuracy of methods for measuring 8-oxo-7,8-dihydroguanine (8-oxoGua) or the nucleoside (8-oxodG). On two occasions, participating laboratories received samples of different concentrations of 8-oxodG for analysis. About half the results returned (for 8-oxodG) were within 20% of the median values. Coefficients of variation (for three identical samples) were commonly around 10%. A sample of calf thymus DNA was sent, dry, to all laboratories. Analysis of 8-oxoGua/8-oxodG in this sample was a test of hydrolysis methods. Almost half the reported results were within 20% of the median value, and half obtained a CV of less than 10%. In order to test sensitivity, as well as precision, DNA was treated with photosensitiser and light to introduce increasing amounts of 8-oxoGua and samples were sent to members. Median values calculated from all returned results were 45.6 (untreated), 53.9, 60.4 and 65.6 8-oxoGua/10(6) Gua; only seven laboratories detected the increase over the whole range, while all but one detected a dose response over two concentration intervals. Results in this trial reflect a continuing improvement in precision and accuracy. The next challenge will be the analysis of 8-oxodG in DNA isolated from cells or tissue, where the concentration is much lower than in calf thymus DNA.",

author = "Collins, {A R} and C Gedik and S Wood and A White and J Dubois and P Duez and Rees, {J F} and R Legall and L Degand and S Loft and A Jensen and H Poulsen and A Weimann and Jensen, {B R} and J Cadet and T Douki and Ravanat, {J L} and H Faure and M Tripier and I Morel and O Sergent and P Cillard and B Morin and B Epe and N Phoa and A Hartwig and A Pelzer and P Dolara and C Casalini and F Guglielmi and C Luceri and H Kasai and R Kido and R Olinski and K Bialkowski and Z Durackova and L Hlincikova and P Korytar and M Dusinska and C Mislanova and J Vina and A Lloret and L Moller and T Hofer and E Gremaud and L Fay and R Stadler and J Eakins and F Pognan and J O'Brien and R Elliott and S Astley and A Bailley and K Herbert and D Chauhan and F Kelly and C Dunster and J Lunec and I Podmore and P Patel and S Johnson and M Evans and R Tyrrell and M Gordon and C Wild and L Hardie and E Smith and Escodd, {[No Value]}",

note = "ID number: ISI:000174725300001",

year = "2002",

doi = "10.1080/10715760290019246",

language = "English",

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pages = "239--245",

journal = "Free Radical Research",

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T1 - Inter-laboratory validation of procedures for measuring 8-oxo-7,8-dihydroguanine/8-oxo-7,8-dihydro-2 '-deoxyguanosine in DNA

AU - Collins, A R

AU - Gedik, C

AU - Wood, S

AU - White, A

AU - Dubois, J

AU - Duez, P

AU - Rees, J F

AU - Legall, R

AU - Degand, L

AU - Loft, S

AU - Jensen, A

AU - Poulsen, H

AU - Weimann, A

AU - Jensen, B R

AU - Cadet, J

AU - Douki, T

AU - Ravanat, J L

AU - Faure, H

AU - Tripier, M

AU - Morel, I

AU - Sergent, O

AU - Cillard, P

AU - Morin, B

AU - Epe, B

AU - Phoa, N

AU - Hartwig, A

AU - Pelzer, A

AU - Dolara, P

AU - Casalini, C

AU - Guglielmi, F

AU - Luceri, C

AU - Kasai, H

AU - Kido, R

AU - Olinski, R

AU - Bialkowski, K

AU - Durackova, Z

AU - Hlincikova, L

AU - Korytar, P

AU - Dusinska, M

AU - Mislanova, C

AU - Vina, J

AU - Lloret, A

AU - Moller, L

AU - Hofer, T

AU - Gremaud, E

AU - Fay, L

AU - Stadler, R

AU - Eakins, J

AU - Pognan, F

AU - O'Brien, J

AU - Elliott, R

AU - Astley, S

AU - Bailley, A

AU - Herbert, K

AU - Chauhan, D

AU - Kelly, F

AU - Dunster, C

AU - Lunec, J

AU - Podmore, I

AU - Patel, P

AU - Johnson, S

AU - Evans, M

AU - Tyrrell, R

AU - Gordon, M

AU - Wild, C

AU - Hardie, L

AU - Smith, E

AU - Escodd, [No Value]

N1 - ID number: ISI:000174725300001

PY - 2002

Y1 - 2002

N2 - The aim of ESCODD, a European Commission funded Concerted Action, is to improve the precision and accuracy of methods for measuring 8-oxo-7,8-dihydroguanine (8-oxoGua) or the nucleoside (8-oxodG). On two occasions, participating laboratories received samples of different concentrations of 8-oxodG for analysis. About half the results returned (for 8-oxodG) were within 20% of the median values. Coefficients of variation (for three identical samples) were commonly around 10%. A sample of calf thymus DNA was sent, dry, to all laboratories. Analysis of 8-oxoGua/8-oxodG in this sample was a test of hydrolysis methods. Almost half the reported results were within 20% of the median value, and half obtained a CV of less than 10%. In order to test sensitivity, as well as precision, DNA was treated with photosensitiser and light to introduce increasing amounts of 8-oxoGua and samples were sent to members. Median values calculated from all returned results were 45.6 (untreated), 53.9, 60.4 and 65.6 8-oxoGua/10(6) Gua; only seven laboratories detected the increase over the whole range, while all but one detected a dose response over two concentration intervals. Results in this trial reflect a continuing improvement in precision and accuracy. The next challenge will be the analysis of 8-oxodG in DNA isolated from cells or tissue, where the concentration is much lower than in calf thymus DNA.

AB - The aim of ESCODD, a European Commission funded Concerted Action, is to improve the precision and accuracy of methods for measuring 8-oxo-7,8-dihydroguanine (8-oxoGua) or the nucleoside (8-oxodG). On two occasions, participating laboratories received samples of different concentrations of 8-oxodG for analysis. About half the results returned (for 8-oxodG) were within 20% of the median values. Coefficients of variation (for three identical samples) were commonly around 10%. A sample of calf thymus DNA was sent, dry, to all laboratories. Analysis of 8-oxoGua/8-oxodG in this sample was a test of hydrolysis methods. Almost half the reported results were within 20% of the median value, and half obtained a CV of less than 10%. In order to test sensitivity, as well as precision, DNA was treated with photosensitiser and light to introduce increasing amounts of 8-oxoGua and samples were sent to members. Median values calculated from all returned results were 45.6 (untreated), 53.9, 60.4 and 65.6 8-oxoGua/10(6) Gua; only seven laboratories detected the increase over the whole range, while all but one detected a dose response over two concentration intervals. Results in this trial reflect a continuing improvement in precision and accuracy. The next challenge will be the analysis of 8-oxodG in DNA isolated from cells or tissue, where the concentration is much lower than in calf thymus DNA.

U2 - 10.1080/10715760290019246

DO - 10.1080/10715760290019246

M3 - Article

SN - 1071-5762

VL - 36

SP - 239

EP - 245

JO - Free Radical Research

JF - Free Radical Research

IS - 3

ER -

Inter-laboratory validation of procedures for measuring 8-oxo-7,8-dihydroguanine/8-oxo-7,8-dihydro-2 '-deoxyguanosine in DNA

Abstract

Bibliographical note

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