Integrated biochip for PCR-based DNA amplification and detection on capacitive biosensors

D. Moschou; N. Vourdas; M. K. Filippidou; V. Tsouti; G. Kokkoris; G. Tsekenis; I. Zergioti; S. Chatzandroulis; A. Tserepi

doi:10.1117/12.2017690

Integrated biochip for PCR-based DNA amplification and detection on capacitive biosensors

D. Moschou
, N. Vourdas
, M. K. Filippidou
, V. Tsouti
, G. Kokkoris
, G. Tsekenis
, I. Zergioti
, S. Chatzandroulis
, A. Tserepi

Department of Electronic & Electrical Engineering

Academy of Athens
National Technical University of Athens
National Centre for Scientific Research “Demokritos”

Research output: Chapter or section in a book/report/conference proceeding › Chapter in a published conference proceeding

18 Citations (SciVal)

Abstract

Responding to an increasing demand for LoC devices to perform bioanalytical protocols for disease diagnostics, the development of an integrated LoC device consisting of a uPCR module integrated with resistive microheaters and a biosensor array for disease diagnostics is presented. The LoC is built on a Printed Circuit Board (PCB) platform, implementing both the amplification of DNA samples and DNA detection/identification on-chip. The resistive microheaters for PCR and the wirings for the sensor read-out are fabricated by means of standard PCB technology. The microfluidic network is continuous-flow, designed to perform 30 PCR cycles with heated zones at constant temperatures, and is built onto the PCB utilizing commercial photopatternable polyimide layers. Following DNA amplification, the product is driven in a chamber where a Si-based biosensor array is placed for DNA detection through hybridization. The sensor array is tested for the detection of mutations of the KRAS gene, responsible for colon cancer.

Original language	English
Title of host publication	Bio-MEMS and Medical Microdevices
Editors	A. Tserepi, M. Delgado-Restituto, E. Makarona
Volume	8765
DOIs	https://doi.org/10.1117/12.2017690
Publication status	Published - 2013
Event	SPIE Microtechnologies BioMEMS and Medical Microdevices Conference - Grenoble, France Duration: 25 Apr 2013 → 26 Apr 2013

Publication series

Name	Proceedings of SPIE
Volume	8765

Conference

Conference	SPIE Microtechnologies BioMEMS and Medical Microdevices Conference
Country/Territory	France
City	Grenoble
Period	25/04/13 → 26/04/13

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Capacitive biosensors
KRAS
Lab-on-Chip
Microfluidics
PCB
UPCR

ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics
Electronic, Optical and Magnetic Materials
Biomaterials
Radiology Nuclear Medicine and imaging

Access to Document

10.1117/12.2017690

Cite this

Moschou, D., Vourdas, N., Filippidou, M. K., Tsouti, V., Kokkoris, G., Tsekenis, G., Zergioti, I., Chatzandroulis, S., & Tserepi, A. (2013). Integrated biochip for PCR-based DNA amplification and detection on capacitive biosensors. In A. Tserepi, M. Delgado-Restituto, & E. Makarona (Eds.), Bio-MEMS and Medical Microdevices (Vol. 8765). Article 87650L (Proceedings of SPIE; Vol. 8765). https://doi.org/10.1117/12.2017690

Integrated biochip for PCR-based DNA amplification and detection on capacitive biosensors. / Moschou, D.; Vourdas, N.; Filippidou, M. K. et al.
Bio-MEMS and Medical Microdevices. ed. / A. Tserepi; M. Delgado-Restituto; E. Makarona. Vol. 8765 2013. 87650L (Proceedings of SPIE; Vol. 8765).

Research output: Chapter or section in a book/report/conference proceeding › Chapter in a published conference proceeding

Moschou, D, Vourdas, N, Filippidou, MK, Tsouti, V, Kokkoris, G, Tsekenis, G, Zergioti, I, Chatzandroulis, S & Tserepi, A 2013, Integrated biochip for PCR-based DNA amplification and detection on capacitive biosensors. in A Tserepi, M Delgado-Restituto & E Makarona (eds), Bio-MEMS and Medical Microdevices. vol. 8765, 87650L, Proceedings of SPIE, vol. 8765, SPIE Microtechnologies BioMEMS and Medical Microdevices Conference, Grenoble, France, 25/04/13. https://doi.org/10.1117/12.2017690

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title = "Integrated biochip for PCR-based DNA amplification and detection on capacitive biosensors",

abstract = "Responding to an increasing demand for LoC devices to perform bioanalytical protocols for disease diagnostics, the development of an integrated LoC device consisting of a uPCR module integrated with resistive microheaters and a biosensor array for disease diagnostics is presented. The LoC is built on a Printed Circuit Board (PCB) platform, implementing both the amplification of DNA samples and DNA detection/identification on-chip. The resistive microheaters for PCR and the wirings for the sensor read-out are fabricated by means of standard PCB technology. The microfluidic network is continuous-flow, designed to perform 30 PCR cycles with heated zones at constant temperatures, and is built onto the PCB utilizing commercial photopatternable polyimide layers. Following DNA amplification, the product is driven in a chamber where a Si-based biosensor array is placed for DNA detection through hybridization. The sensor array is tested for the detection of mutations of the KRAS gene, responsible for colon cancer.",

keywords = "Capacitive biosensors, KRAS, Lab-on-Chip, Microfluidics, PCB, UPCR",

author = "D. Moschou and N. Vourdas and Filippidou, \{M. K.\} and V. Tsouti and G. Kokkoris and G. Tsekenis and I. Zergioti and S. Chatzandroulis and A. Tserepi",

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AU - Moschou, D.

AU - Vourdas, N.

AU - Filippidou, M. K.

AU - Tsouti, V.

AU - Kokkoris, G.

AU - Tsekenis, G.

AU - Zergioti, I.

AU - Chatzandroulis, S.

AU - Tserepi, A.

PY - 2013

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N2 - Responding to an increasing demand for LoC devices to perform bioanalytical protocols for disease diagnostics, the development of an integrated LoC device consisting of a uPCR module integrated with resistive microheaters and a biosensor array for disease diagnostics is presented. The LoC is built on a Printed Circuit Board (PCB) platform, implementing both the amplification of DNA samples and DNA detection/identification on-chip. The resistive microheaters for PCR and the wirings for the sensor read-out are fabricated by means of standard PCB technology. The microfluidic network is continuous-flow, designed to perform 30 PCR cycles with heated zones at constant temperatures, and is built onto the PCB utilizing commercial photopatternable polyimide layers. Following DNA amplification, the product is driven in a chamber where a Si-based biosensor array is placed for DNA detection through hybridization. The sensor array is tested for the detection of mutations of the KRAS gene, responsible for colon cancer.

AB - Responding to an increasing demand for LoC devices to perform bioanalytical protocols for disease diagnostics, the development of an integrated LoC device consisting of a uPCR module integrated with resistive microheaters and a biosensor array for disease diagnostics is presented. The LoC is built on a Printed Circuit Board (PCB) platform, implementing both the amplification of DNA samples and DNA detection/identification on-chip. The resistive microheaters for PCR and the wirings for the sensor read-out are fabricated by means of standard PCB technology. The microfluidic network is continuous-flow, designed to perform 30 PCR cycles with heated zones at constant temperatures, and is built onto the PCB utilizing commercial photopatternable polyimide layers. Following DNA amplification, the product is driven in a chamber where a Si-based biosensor array is placed for DNA detection through hybridization. The sensor array is tested for the detection of mutations of the KRAS gene, responsible for colon cancer.

KW - Capacitive biosensors

KW - KRAS

KW - Lab-on-Chip

KW - Microfluidics

KW - PCB

KW - UPCR

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M3 - Chapter in a published conference proceeding

AN - SCOPUS:84881140897

SN - 9780819495624

VL - 8765

T3 - Proceedings of SPIE

BT - Bio-MEMS and Medical Microdevices

A2 - Tserepi, A.

A2 - Delgado-Restituto, M.

A2 - Makarona, E.

T2 - SPIE Microtechnologies BioMEMS and Medical Microdevices Conference

Y2 - 25 April 2013 through 26 April 2013

ER -

Integrated biochip for PCR-based DNA amplification and detection on capacitive biosensors

Abstract

Publication series

Conference

UN SDGs

Keywords

ASJC Scopus subject areas

Access to Document

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