Integrated biochip for PCR-based DNA amplification and detection on capacitive biosensors

D. Moschou, N. Vourdas, M. K. Filippidou, V. Tsouti, G. Kokkoris, G. Tsekenis, I. Zergioti, S. Chatzandroulis, A. Tserepi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

12 Citations (Scopus)

Abstract

Responding to an increasing demand for LoC devices to perform bioanalytical protocols for disease diagnostics, the development of an integrated LoC device consisting of a uPCR module integrated with resistive microheaters and a biosensor array for disease diagnostics is presented. The LoC is built on a Printed Circuit Board (PCB) platform, implementing both the amplification of DNA samples and DNA detection/identification on-chip. The resistive microheaters for PCR and the wirings for the sensor read-out are fabricated by means of standard PCB technology. The microfluidic network is continuous-flow, designed to perform 30 PCR cycles with heated zones at constant temperatures, and is built onto the PCB utilizing commercial photopatternable polyimide layers. Following DNA amplification, the product is driven in a chamber where a Si-based biosensor array is placed for DNA detection through hybridization. The sensor array is tested for the detection of mutations of the KRAS gene, responsible for colon cancer.

Original languageEnglish
Title of host publicationBio-MEMS and Medical Microdevices
EditorsA. Tserepi, M. Delgado-Restituto, E. Makarona
Volume8765
DOIs
Publication statusPublished - 2013
EventSPIE Microtechnologies BioMEMS and Medical Microdevices Conference - Grenoble, France
Duration: 25 Apr 201326 Apr 2013

Publication series

NameProceedings of SPIE
Volume8765

Conference

ConferenceSPIE Microtechnologies BioMEMS and Medical Microdevices Conference
CountryFrance
CityGrenoble
Period25/04/1326/04/13

Keywords

  • Capacitive biosensors
  • KRAS
  • Lab-on-Chip
  • Microfluidics
  • PCB
  • UPCR

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

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