Initial characterization of the enzyme and cloning of genes involved in the enantioselective epoxyalkane degradation by xanthobacter PY2

J. Swaving; C. A G M Weijers; C. K Chan Kwo Chion; D. J. Leak; A. J J Van Ooyen; J. A M De Bont

doi:10.3109/10242429409065232

Initial characterization of the enzyme and cloning of genes involved in the enantioselective epoxyalkane degradation by xanthobacter PY2

J. Swaving, C. A G M Weijers, C. K Chan Kwo Chion, D. J. Leak, A. J J Van Ooyen, J. A M De Bont

Research output: Contribution to journal › Article › peer-review

Abstract

The epoxyalkane-degrading enzyme of Xanthobacter Py2 was studied in crude extracts. NAD and a low molecular weight fraction which can be replaced by DTT are necessary for the conversion of epoxyalkanes into ketones. The genes responsible for the epoxyalkane-degrading enzyme were cloned and expressed in Xanthobacter autotrophics GJ10.

Original language	English
Pages (from-to)	227-232
Number of pages	6
Journal	Biocatalysis and Biotransformation
Volume	10
Issue number	1-4
DOIs	https://doi.org/10.3109/10242429409065232
Publication status	Published - 1 Jan 1994

Funding

This study was supported by a grant from the European Community (BIOT- CT9 1-0269).

Keywords

Epoxides
Epoxyalkane degradation
Xanthobacter Py2

ASJC Scopus subject areas

Biochemistry
Biotechnology
Catalysis

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10.3109/10242429409065232

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abstract = "The epoxyalkane-degrading enzyme of Xanthobacter Py2 was studied in crude extracts. NAD and a low molecular weight fraction which can be replaced by DTT are necessary for the conversion of epoxyalkanes into ketones. The genes responsible for the epoxyalkane-degrading enzyme were cloned and expressed in Xanthobacter autotrophics GJ10.",

keywords = "Epoxides, Epoxyalkane degradation, Xanthobacter Py2",

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AU - Chion, C. K Chan Kwo

AU - Leak, D. J.

AU - Ooyen, A. J J Van

AU - Bont, J. A M De

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AB - The epoxyalkane-degrading enzyme of Xanthobacter Py2 was studied in crude extracts. NAD and a low molecular weight fraction which can be replaced by DTT are necessary for the conversion of epoxyalkanes into ketones. The genes responsible for the epoxyalkane-degrading enzyme were cloned and expressed in Xanthobacter autotrophics GJ10.

KW - Epoxides

KW - Epoxyalkane degradation

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Initial characterization of the enzyme and cloning of genes involved in the enantioselective epoxyalkane degradation by xanthobacter PY2

Abstract

Funding

Keywords

ASJC Scopus subject areas

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