Initial characterization of the enzyme and cloning of genes involved in the enantioselective epoxyalkane degradation by xanthobacter PY2

J. Swaving, C. A G M Weijers, C. K Chan Kwo Chion, D. J. Leak, A. J J Van Ooyen, J. A M De Bont

Research output: Contribution to journalArticle

Abstract

The epoxyalkane-degrading enzyme of Xanthobacter Py2 was studied in crude extracts. NAD and a low molecular weight fraction which can be replaced by DTT are necessary for the conversion of epoxyalkanes into ketones. The genes responsible for the epoxyalkane-degrading enzyme were cloned and expressed in Xanthobacter autotrophics GJ10.

Original languageEnglish
Pages (from-to)227-232
Number of pages6
JournalBiocatalysis and Biotransformation
Volume10
Issue number1-4
DOIs
Publication statusPublished - 1 Jan 1994

Keywords

  • Epoxides
  • Epoxyalkane degradation
  • Xanthobacter Py2

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Catalysis

Cite this

Initial characterization of the enzyme and cloning of genes involved in the enantioselective epoxyalkane degradation by xanthobacter PY2. / Swaving, J.; Weijers, C. A G M; Chion, C. K Chan Kwo; Leak, D. J.; Ooyen, A. J J Van; Bont, J. A M De.

In: Biocatalysis and Biotransformation, Vol. 10, No. 1-4, 01.01.1994, p. 227-232.

Research output: Contribution to journalArticle

Swaving, J. ; Weijers, C. A G M ; Chion, C. K Chan Kwo ; Leak, D. J. ; Ooyen, A. J J Van ; Bont, J. A M De. / Initial characterization of the enzyme and cloning of genes involved in the enantioselective epoxyalkane degradation by xanthobacter PY2. In: Biocatalysis and Biotransformation. 1994 ; Vol. 10, No. 1-4. pp. 227-232.
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