Initial characterization of the enzyme and cloning of genes involved in the enantioselective epoxyalkane degradation by xanthobacter PY2

J. Swaving, C. A G M Weijers, C. K Chan Kwo Chion, D. J. Leak, A. J J Van Ooyen, J. A M De Bont

Research output: Contribution to journalArticle

Abstract

The epoxyalkane-degrading enzyme of Xanthobacter Py2 was studied in crude extracts. NAD and a low molecular weight fraction which can be replaced by DTT are necessary for the conversion of epoxyalkanes into ketones. The genes responsible for the epoxyalkane-degrading enzyme were cloned and expressed in Xanthobacter autotrophics GJ10.

Original languageEnglish
Pages (from-to)227-232
Number of pages6
JournalBiocatalysis and Biotransformation
Volume10
Issue number1-4
DOIs
Publication statusPublished - 1 Jan 1994

Keywords

  • Epoxides
  • Epoxyalkane degradation
  • Xanthobacter Py2

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Catalysis

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