Cycling level was investigated for four cities with similar land area namely, Beijing, Berlin, Canberra and Singapore. Four characteristics namely, urban structure, transport policy, public transport service and cycling infrastructure, as affecting cycling activities in urban areas were compared. A cycling line was devised and used to position and compare each city by the stages of cycling level. Cycling level in Berlin is comparatively high and remains on an increasing trend which is at the promising stage. Recommendations are given for the other three cities (Beijing, Canberra and Singapore) that could improve cycling level.