Influence of reaction-induced convection on quorum sensing in enzyme-loaded agarose beads

Vladimir M. Markovic; Tamás Bánsági; Dennel McKenzie; Anthony Mai; John A. Pojman; Annette F. Taylor

doi:10.1063/1.5089295

Influence of reaction-induced convection on quorum sensing in enzyme-loaded agarose beads

Vladimir M. Markovic, Tamás Bánsági, Dennel McKenzie, Anthony Mai, John A. Pojman, Annette F. Taylor

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

9 Citations (SciVal)

Abstract

In theory, groups of enzyme-loaded particles producing an acid or base may show complex behavior including dynamical quorum sensing, the appearance of synchronized oscillations above a critical number or density of particles. Here, experiments were performed with the enzyme urease loaded into mm-sized agarose beads and placed in a solution of urea, resulting in an increase in pH. This behavior was found to be dependent upon the number of beads present in the array; however, reaction-induced convection occurred and plumes of high pH developed that extended to the walls of the reactor. The convection resulted in the motion of the mm-sized particles and conversion of the solution to high pH. Simulations in a simple model of the beads demonstrated the suppression of dynamical quorum sensing in the presence of flow.

Original language	English
Article number	033130
Journal	Chaos
Volume	29
Issue number	3
Early online date	26 Mar 2019
DOIs	https://doi.org/10.1063/1.5089295
Publication status	Published - 31 Mar 2019

Bibliographical note

Funding Information:
V.M.M. thanks COST Action CM1304 Emergence and Evolution in Complex Chemical Systems for funding a Short-Term Scientific Mission to the University of She eld and also thanks the Ministry of Education, Science and Technological Development of the Republic of Serbia (Grant No. 172015); A.F.T. and T.B. thank Engineering and Physical Sciences Research Consul (EPSRC) (Grant No. EP/K030574/2) for financial support.

Publisher Copyright:
© 2019 Author(s).

ASJC Scopus subject areas

Statistical and Nonlinear Physics
Mathematical Physics
General Physics and Astronomy
Applied Mathematics

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title = "Influence of reaction-induced convection on quorum sensing in enzyme-loaded agarose beads",

abstract = "In theory, groups of enzyme-loaded particles producing an acid or base may show complex behavior including dynamical quorum sensing, the appearance of synchronized oscillations above a critical number or density of particles. Here, experiments were performed with the enzyme urease loaded into mm-sized agarose beads and placed in a solution of urea, resulting in an increase in pH. This behavior was found to be dependent upon the number of beads present in the array; however, reaction-induced convection occurred and plumes of high pH developed that extended to the walls of the reactor. The convection resulted in the motion of the mm-sized particles and conversion of the solution to high pH. Simulations in a simple model of the beads demonstrated the suppression of dynamical quorum sensing in the presence of flow.",

author = "Markovic, {Vladimir M.} and Tam{\'a}s B{\'a}ns{\'a}gi and Dennel McKenzie and Anthony Mai and Pojman, {John A.} and Taylor, {Annette F.}",

note = "Funding Information: V.M.M. thanks COST Action CM1304 Emergence and Evolution in Complex Chemical Systems for funding a Short-Term Scientific Mission to the University of She eld and also thanks the Ministry of Education, Science and Technological Development of the Republic of Serbia (Grant No. 172015); A.F.T. and T.B. thank Engineering and Physical Sciences Research Consul (EPSRC) (Grant No. EP/K030574/2) for financial support. Publisher Copyright: {\textcopyright} 2019 Author(s).",

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AU - Markovic, Vladimir M.

AU - Bánsági, Tamás

AU - McKenzie, Dennel

AU - Mai, Anthony

AU - Pojman, John A.

AU - Taylor, Annette F.

N1 - Funding Information: V.M.M. thanks COST Action CM1304 Emergence and Evolution in Complex Chemical Systems for funding a Short-Term Scientific Mission to the University of She eld and also thanks the Ministry of Education, Science and Technological Development of the Republic of Serbia (Grant No. 172015); A.F.T. and T.B. thank Engineering and Physical Sciences Research Consul (EPSRC) (Grant No. EP/K030574/2) for financial support. Publisher Copyright: © 2019 Author(s).

PY - 2019/3/31

Y1 - 2019/3/31

N2 - In theory, groups of enzyme-loaded particles producing an acid or base may show complex behavior including dynamical quorum sensing, the appearance of synchronized oscillations above a critical number or density of particles. Here, experiments were performed with the enzyme urease loaded into mm-sized agarose beads and placed in a solution of urea, resulting in an increase in pH. This behavior was found to be dependent upon the number of beads present in the array; however, reaction-induced convection occurred and plumes of high pH developed that extended to the walls of the reactor. The convection resulted in the motion of the mm-sized particles and conversion of the solution to high pH. Simulations in a simple model of the beads demonstrated the suppression of dynamical quorum sensing in the presence of flow.

AB - In theory, groups of enzyme-loaded particles producing an acid or base may show complex behavior including dynamical quorum sensing, the appearance of synchronized oscillations above a critical number or density of particles. Here, experiments were performed with the enzyme urease loaded into mm-sized agarose beads and placed in a solution of urea, resulting in an increase in pH. This behavior was found to be dependent upon the number of beads present in the array; however, reaction-induced convection occurred and plumes of high pH developed that extended to the walls of the reactor. The convection resulted in the motion of the mm-sized particles and conversion of the solution to high pH. Simulations in a simple model of the beads demonstrated the suppression of dynamical quorum sensing in the presence of flow.

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Influence of reaction-induced convection on quorum sensing in enzyme-loaded agarose beads

Abstract

Bibliographical note

ASJC Scopus subject areas

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