Inexpensive and fast pathogenic bacteria screening using field-effect transistors

Nello Formisano, Nikhil Bhalla, Mel Heeran, Juana Reyes Martinez, Amrita Sarkar, Maisem Laabei, Pawan Jolly, Chris R. Bowen, John T. Taylor, Sabine Flitsch, Pedro Estrela

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

While pathogenic bacteria contribute to a large number of globally important diseases and infections, current clinical diagnosis is based on processes that last a few days, employing complex and expensive techniques. Therefore, innovative, simple, rapid and low-cost solutions to effectively reduce the burden of bacterial infections are urgently needed. Here we demonstrate a label-free sensor for fast bacterial detection based on metal–oxide–semiconductor field-effect transistors (MOSFETs). The electric charge of bacteria binding to the glycosylated gates of a MOSFET enables quantification in a straightforward manner. We show that the limit of quantitation is 1.9 × 105 CFU/ml with this simple device, which is more than 10,000-times lower than is possible with existing techniques such as matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-ToF) on the same modified surfaces. Moreover, the measurements are extremely fast and the sensor can be mass produced at trivial cost as a tool for initial screening of pathogens.
LanguageEnglish
Pages103-109
Number of pages7
JournalBiosensors and Bioelectronics
Volume85
Early online date21 Apr 2016
DOIs
StatusPublished - 15 Nov 2016

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Semiconductors
MOSFET devices
Field effect transistors
Oxides
Bacteria
Screening
Metals
Costs and Cost Analysis
Electric charge
Sensors
Pathogens
Bacterial Infections
Ionization
Mass spectrometry
Labels
Costs
Desorption
Mass Spectrometry
Lasers
Equipment and Supplies

Cite this

Inexpensive and fast pathogenic bacteria screening using field-effect transistors. / Formisano, Nello; Bhalla, Nikhil; Heeran, Mel; Reyes Martinez, Juana; Sarkar, Amrita; Laabei, Maisem; Jolly, Pawan; Bowen, Chris R.; Taylor, John T.; Flitsch, Sabine; Estrela, Pedro.

In: Biosensors and Bioelectronics, Vol. 85, 15.11.2016, p. 103-109.

Research output: Contribution to journalArticle

Formisano, Nello ; Bhalla, Nikhil ; Heeran, Mel ; Reyes Martinez, Juana ; Sarkar, Amrita ; Laabei, Maisem ; Jolly, Pawan ; Bowen, Chris R. ; Taylor, John T. ; Flitsch, Sabine ; Estrela, Pedro. / Inexpensive and fast pathogenic bacteria screening using field-effect transistors. In: Biosensors and Bioelectronics. 2016 ; Vol. 85. pp. 103-109.
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