Individual p KaValues of Tobramycin, Kanamycin B, Amikacin, Sisomicin, and Netilmicin Determined by Multinuclear NMR Spectroscopy

Abdulaziz H. Alkhzem, Timothy J. Woodman, Ian S. Blagbrough

Research output: Contribution to journalArticle

Abstract

NMR spectroscopy is a powerful technique for separating and measuring each distinct pKa value of the amino groups around aminoglycoside antibiotics. Unambiguous assignments were made for each individual amine substituent on 2-deoxystreptamine, tobramycin, kanamycin B, amikacin, sisomicin, and netilmicin using variations in the NMR spectroscopic chemical shift (δ) with 1H, 13C, and 15N HMBC; the individual pKa values of netilmicin are reported for the first time.

Original languageEnglish
Pages (from-to)21094–21103
JournalACS OMEGA
Volume5
Issue number33
Early online date11 Aug 2020
DOIs
Publication statusPublished - 25 Aug 2020

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

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