Electrochemical measurements using nonconducting support materials, such as paper and cotton gauze, for modifying glassy carbon (GC) with gold nanoparticles (AuNPs) are described. After a seed-mediated growth modification of AuNPs on Kimwipes, a AuNP-Kimwipes-modified GC electrode that was fabricated by simply fixing a piece of AuNP-modified Kimwipes on a GC electrode surface showed almost reversible cyclic voltammetric responses for the oxidation of Fe(CN)64- in 0.1M phosphate buffer solution (pH7.0) even when the GC surface was not polished before fixing. The electrochemical impedance results also indicated the significant effect of the AuNP-modified Kimwipes on reducing the charge transfer resistance. Similar effects were observed for AuNP-modified cotton gauze, whose characteristics were the partial coverage of a GC surface, and, for the electrode in that a piece of untreated Kimwipes was bound between AuNP-modified Kimwipes and a GC electrode surface. On the basis of such partial or remote effects of AuNPs together with other electrochemical results, plausible mechanisms to promote the electron transfer reaction of Fe(CN)64- by AuNPs are discussed. In addition, as a specific phenomenon in the present modified electrodes, the adsorption of Fe(CN)64- with the presence of AuNPs is described.