Incidence of endornaviruses in Phytophthora taxon douglasfir and Phytophthora ramorum

Zisis Kozlakidis, Neil A. Brown, Atif Jamal, Xiyu Phoon, Robert H.A. Coutts

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

In this investigation, we show that four Phytophthora taxon douglasfir isolates from the USA, irrespective of their geographical location or host plant, and 20% of a representative cohort of Phytophthora ramorum isolates contain endornavirus dsRNAs. Three endornavirus-specific RT-PCR amplicons were generated by RT-PCR using dsRNA isolated from the four Phytophthora taxon douglasfir isolates and one representative Phytophthora ramorum isolate as template with oligonucleotide primers designed from the sequence of Phytophthora endornavirus 1. The amplified segments showed a very high degree of sequence similarity suggesting that the virus has gone through a population bottleneck during its emergence.

Original languageEnglish
Pages (from-to)130-134
Number of pages5
JournalVirus Genes
Volume40
Issue number1
DOIs
Publication statusPublished - 1 Feb 2010

Keywords

  • DsRNA
  • Endornavirus
  • Phytophthora spp.

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Virology

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