In vivo multiphoton microscopy using a handheld scanner with lateral and axial motion compensation

Ben Sherlock, Chris Dunsby, Jonathan Knight, Fei Yu, James Stone, Carl Paterson, Paul French, Mark Neil, Sean Warren, Yuiri Alexansrov

Research output: Contribution to journalArticlepeer-review

11 Citations (SciVal)

Abstract

This paper reports a handheld multiphoton fluorescence microscope designed for
clinical imaging that incorporates axial motion compensation and lateral image
stabilization. Spectral domain optical coherence tomography is employed to track the axial position of the skin surface, and lateral motion compensation is realised by imaging the speckle pattern arising from the optical coherence tomography beam illuminating the sample. Combined with the use of negative curvature microstructured optical fibre to deliver tunable ultrafast radiation to the handheld multiphoton scanner without the need of a dispersion compensation unit, this instrument has potential for a range of clinical applications. The system is used to compensate for both lateral and
axial motion of the sample when imaging human skin in vivo.
Original languageEnglish
JournalJournal of Biophotonics
Early online date31 Aug 2017
DOIs
Publication statusE-pub ahead of print - 31 Aug 2017

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