In vitro reprogramming of pancreatic cells to hepatocytes

Daniel Eberhard, Kathy O’Neill, Zoe D Burke, David Tosh

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Citations (Scopus)

Abstract

Transdifferentiation is defined as the conversion of one cell type to another. One well-documented example of transdifferentiation is the conversion of pancreatic cells to hepatocytes. Here we describe a robust in vitro model to study pancreas to liver transdifferentiation. It is based on the addition of the synthetic glucocorticoid dexamethasone to the rat pancreatic exocrine cell line AR42J. Following glucocorticoid treatment, cells resembling hepatocytes arc induced. Transdifferentiated hepatocytes express many of the properties of bona fide hepatocytcs, e.g. production of albumin and ability to respond to xenobiotics. These hepatocytes can be used for studying liver function in vitro as well as studying the molecular basis of transdifferentiation.
Original languageEnglish
Title of host publicationCellular Programming and Reprogramming: Methods and Protocols
EditorsS Ding
PublisherHumana Press
Pages285-292
Number of pages8
Edition636
ISBN (Print)9781607616900
DOIs
Publication statusPublished - 2010

Publication series

NameMethods in Molecular Biology
PublisherHumana Press

Fingerprint

Hepatocytes
Glucocorticoids
Liver
Xenobiotics
Dexamethasone
Pancreas
Albumins
Cell Line
In Vitro Techniques
Cellular Reprogramming

Cite this

Eberhard, D., O’Neill, K., Burke, Z. D., & Tosh, D. (2010). In vitro reprogramming of pancreatic cells to hepatocytes. In S. Ding (Ed.), Cellular Programming and Reprogramming: Methods and Protocols (636 ed., pp. 285-292). (Methods in Molecular Biology). Humana Press. https://doi.org/10.1007/978-1-60761-691-7_17

In vitro reprogramming of pancreatic cells to hepatocytes. / Eberhard, Daniel; O’Neill, Kathy; Burke, Zoe D; Tosh, David.

Cellular Programming and Reprogramming: Methods and Protocols. ed. / S Ding. 636. ed. Humana Press, 2010. p. 285-292 (Methods in Molecular Biology).

Research output: Chapter in Book/Report/Conference proceedingChapter

Eberhard, D, O’Neill, K, Burke, ZD & Tosh, D 2010, In vitro reprogramming of pancreatic cells to hepatocytes. in S Ding (ed.), Cellular Programming and Reprogramming: Methods and Protocols. 636 edn, Methods in Molecular Biology, Humana Press, pp. 285-292. https://doi.org/10.1007/978-1-60761-691-7_17
Eberhard D, O’Neill K, Burke ZD, Tosh D. In vitro reprogramming of pancreatic cells to hepatocytes. In Ding S, editor, Cellular Programming and Reprogramming: Methods and Protocols. 636 ed. Humana Press. 2010. p. 285-292. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-60761-691-7_17
Eberhard, Daniel ; O’Neill, Kathy ; Burke, Zoe D ; Tosh, David. / In vitro reprogramming of pancreatic cells to hepatocytes. Cellular Programming and Reprogramming: Methods and Protocols. editor / S Ding. 636. ed. Humana Press, 2010. pp. 285-292 (Methods in Molecular Biology).
@inbook{ee095bc54744400db36d727c1c277683,
title = "In vitro reprogramming of pancreatic cells to hepatocytes",
abstract = "Transdifferentiation is defined as the conversion of one cell type to another. One well-documented example of transdifferentiation is the conversion of pancreatic cells to hepatocytes. Here we describe a robust in vitro model to study pancreas to liver transdifferentiation. It is based on the addition of the synthetic glucocorticoid dexamethasone to the rat pancreatic exocrine cell line AR42J. Following glucocorticoid treatment, cells resembling hepatocytes arc induced. Transdifferentiated hepatocytes express many of the properties of bona fide hepatocytcs, e.g. production of albumin and ability to respond to xenobiotics. These hepatocytes can be used for studying liver function in vitro as well as studying the molecular basis of transdifferentiation.",
author = "Daniel Eberhard and Kathy O’Neill and Burke, {Zoe D} and David Tosh",
year = "2010",
doi = "10.1007/978-1-60761-691-7_17",
language = "English",
isbn = "9781607616900",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "285--292",
editor = "S Ding",
booktitle = "Cellular Programming and Reprogramming: Methods and Protocols",
address = "USA United States",
edition = "636",

}

TY - CHAP

T1 - In vitro reprogramming of pancreatic cells to hepatocytes

AU - Eberhard, Daniel

AU - O’Neill, Kathy

AU - Burke, Zoe D

AU - Tosh, David

PY - 2010

Y1 - 2010

N2 - Transdifferentiation is defined as the conversion of one cell type to another. One well-documented example of transdifferentiation is the conversion of pancreatic cells to hepatocytes. Here we describe a robust in vitro model to study pancreas to liver transdifferentiation. It is based on the addition of the synthetic glucocorticoid dexamethasone to the rat pancreatic exocrine cell line AR42J. Following glucocorticoid treatment, cells resembling hepatocytes arc induced. Transdifferentiated hepatocytes express many of the properties of bona fide hepatocytcs, e.g. production of albumin and ability to respond to xenobiotics. These hepatocytes can be used for studying liver function in vitro as well as studying the molecular basis of transdifferentiation.

AB - Transdifferentiation is defined as the conversion of one cell type to another. One well-documented example of transdifferentiation is the conversion of pancreatic cells to hepatocytes. Here we describe a robust in vitro model to study pancreas to liver transdifferentiation. It is based on the addition of the synthetic glucocorticoid dexamethasone to the rat pancreatic exocrine cell line AR42J. Following glucocorticoid treatment, cells resembling hepatocytes arc induced. Transdifferentiated hepatocytes express many of the properties of bona fide hepatocytcs, e.g. production of albumin and ability to respond to xenobiotics. These hepatocytes can be used for studying liver function in vitro as well as studying the molecular basis of transdifferentiation.

UR - http://dx.doi.org/10.1007/978-1-60761-691-7_17

U2 - 10.1007/978-1-60761-691-7_17

DO - 10.1007/978-1-60761-691-7_17

M3 - Chapter

SN - 9781607616900

T3 - Methods in Molecular Biology

SP - 285

EP - 292

BT - Cellular Programming and Reprogramming: Methods and Protocols

A2 - Ding, S

PB - Humana Press

ER -