In Situ Quenching of Trialkylphosphine Reducing Agents Using Water-Soluble PEG-Azides Improves Maleimide Conjugation to Proteins

Terrence Kantner, Bayan Alkhawaja, Andrew Watts

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)
203 Downloads (Pure)

Abstract

Trialkylphosphines tris(2-carboxy-ethyl)-phosphine and tris(3-hydroxypropyl)-
phosphine are popular reagents for the reduction of cysteine residues in bioconjugation reactions using maleimides. However, it has been demonstrated that these phosphines are reactive toward maleimide, necessitating their removal before the addition of the Michael acceptor. Here, a method using water-soluble PEG-azides is reported for the quenching of trialkylphosphines in situ, which is demonstrated to improve the level of maleimide conjugation to proteins.
Original languageEnglish
Pages (from-to)5785-5791
Number of pages7
JournalACS OMEGA
Volume2
Issue number9
Early online date14 Sep 2017
DOIs
Publication statusPublished - 30 Sep 2017

Fingerprint Dive into the research topics of 'In Situ Quenching of Trialkylphosphine Reducing Agents Using Water-Soluble PEG-Azides Improves Maleimide Conjugation to Proteins'. Together they form a unique fingerprint.

Cite this