Impedimetric paper-based biosensor for the detection of bacterial contamination in water

Saravanan Rengaraj, Alvaro Cruz-Izquierdo, Janet Scott, Mirella Di Lorenzo

Research output: Contribution to journalArticle

16 Citations (Scopus)
65 Downloads (Pure)

Abstract

According to the World Health Organisation, worldwide waterborne diseases are responsible for nearly two million human deaths annually. Rapid and at-site screen of pathogenic microorganisms in drinking water can help to markedly reduce this number. Here we report an innovative, simple and low-cost, paper-based probe for detection of bacteria in water, fabricated by screen printing carbon electrodes onto hydrophobic paper. Electrochemical characterization of the printed electrodes confirmed fast-electron transfer, with an estimated electroactive surface area of 0.25 cm 2. The electrode surface was functionalised with carboxyl groups, prior to covalent immobilization of the lectin Concanavalin A (Con A), used as the biorecognition element. The system was then tested as an impedimetric sensor for bacteria in water. A linear increase in the probe charge transfer resistance was observed for bacterial concentrations ranging from 10 3 to 10 6 CFU mL −1, with an estimated lower detection limit of 1.9 × 10 3 CFU mL −1. Considering its remarkable simplicity, cost-effectiveness and biodegradability, the sensor here reported could be an attractive solution for portable testing kits that address the challenges of traditional time-consuming and expensive lab-based analyses.

Original languageEnglish
Pages (from-to)50-58
Number of pages9
JournalSensors and Actuators B: Chemical
Volume265
Early online date7 Mar 2018
DOIs
Publication statusPublished - 15 Jul 2018

Fingerprint

bioinstrumentation
Biosensors
contamination
Contamination
bacteria
Electrodes
electrodes
Water
Bacteria
water
biodegradability
cost effectiveness
kits
drinking
Screen printing
probes
Biodegradability
sensors
Sensors
carboxyl group

Keywords

  • Electrochemical impedance spectroscopy
  • Lectin-bacteria complex
  • Paper electronics
  • Screen printed electrode

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

Cite this

Impedimetric paper-based biosensor for the detection of bacterial contamination in water. / Rengaraj, Saravanan; Cruz-Izquierdo, Alvaro; Scott, Janet; Di Lorenzo, Mirella.

In: Sensors and Actuators B: Chemical, Vol. 265, 15.07.2018, p. 50-58.

Research output: Contribution to journalArticle

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