Projects per year
Abstract
According to the World Health Organisation, worldwide waterborne diseases are responsible for nearly two million human deaths annually. Rapid and at-site screen of pathogenic microorganisms in drinking water can help to markedly reduce this number. Here we report an innovative, simple and low-cost, paper-based probe for detection of bacteria in water, fabricated by screen printing carbon electrodes onto hydrophobic paper. Electrochemical characterization of the printed electrodes confirmed fast-electron transfer, with an estimated electroactive surface area of 0.25 cm 2. The electrode surface was functionalised with carboxyl groups, prior to covalent immobilization of the lectin Concanavalin A (Con A), used as the biorecognition element. The system was then tested as an impedimetric sensor for bacteria in water. A linear increase in the probe charge transfer resistance was observed for bacterial concentrations ranging from 10 3 to 10 6 CFU mL −1, with an estimated lower detection limit of 1.9 × 10 3 CFU mL −1. Considering its remarkable simplicity, cost-effectiveness and biodegradability, the sensor here reported could be an attractive solution for portable testing kits that address the challenges of traditional time-consuming and expensive lab-based analyses.
Original language | English |
---|---|
Pages (from-to) | 50-58 |
Number of pages | 9 |
Journal | Sensors and Actuators B: Chemical |
Volume | 265 |
Early online date | 7 Mar 2018 |
DOIs | |
Publication status | Published - 15 Jul 2018 |
Keywords
- Electrochemical impedance spectroscopy
- Lectin-bacteria complex
- Paper electronics
- Screen printed electrode
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Instrumentation
- Condensed Matter Physics
- Surfaces, Coatings and Films
- Metals and Alloys
- Electrical and Electronic Engineering
- Materials Chemistry
Fingerprint
Dive into the research topics of 'Impedimetric paper-based biosensor for the detection of bacterial contamination in water'. Together they form a unique fingerprint.Projects
- 1 Finished
-
Vaccinating the Nexus
Di Lorenzo, M. (PI)
Engineering and Physical Sciences Research Council
1/10/15 → 31/05/19
Project: Research council
Profiles
-
Mirella Di Lorenzo
- Department of Chemical Engineering - Professor
- Faculty of Engineering and Design - Associate Dean (International)
- Centre for Sustainable Chemical Technologies (CSCT)
- Water Innovation and Research Centre (WIRC)
- Institute of Sustainability and Climate Change
- Centre for Bioengineering & Biomedical Technologies (CBio)
- Bath Institute for the Augmented Human
- Centre of Excellence in Water-Based Early-Warning Systems for Health Protection (CWBE)
Person: Research & Teaching, Core staff
Equipment
-
-
-
MC2-Electron Microscopy (EM)
Material and Chemical Characterisation (MC2)Facility/equipment: Technology type