Immune anticipation of mating in Drosophila

Turandot M promotes immunity against sexually transmitted fungal infections

Weihao Zhong, Colin Mcclure, Cara Evans, David Mlynski, Elina Immonen, Michael Ritchie, N K Priest

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Although it is well known that mating increases the risk of infection, we do not know how females mitigate the fitness costs of sexually transmitted infections (STIs). It has recently been shown that female fruitflies, Drosophila melanogaster, specifically upregulate two members of the Turandot family of immune and stress response genes, Turandot M and Turandot C (TotM and TotC), when they hear male courtship song. Here, we use the Gal4/UAS RNAi gene knockdown system to test whether the expression of these genes provides fitness benefits for females infected with the entomopathogenic fungus, Metarhizium robertsii under sexual transmission. As a control, we also examined the immunity conferred by Dorsal-related immunity factor (Dif), a central component of the Toll signalling pathway thought to provide immunity against fungal infections. We show that TotM, but not TotC or Dif, provides survival benefits to females following STIs, but not after direct topical infections. We also show that though the expression of TotM provides fecundity benefits for healthy females, it comes at a cost to their survival, which helps to explain why TotM is not constitutively expressed. Together, these results show that the anticipatory expression of TotM promotes specific immunity against fungal STIs and suggest that immune anticipation is more common than currently appreciated.
Original languageEnglish
Article number20132018
Number of pages9
JournalProceedings of the Royal Society B: Biological Sciences
Volume280
Issue number1773
Early online date30 Oct 2013
DOIs
Publication statusPublished - 22 Dec 2013

Fingerprint

Mycoses
Sexually Transmitted Diseases
immunity
Drosophila
Immunity
sexually transmitted diseases
Genes
infection
Fungi
Metarhizium
gene
Costs
Gene Knockdown Techniques
fitness
Courtship
Costs and Cost Analysis
entomopathogenic fungi
Music
fruit flies
RNA Interference

Keywords

  • immune anticipation
  • innate immunity
  • ecological immunology
  • sexually transmitted infections
  • Metarhizium robertsii
  • Drosophila melanogaster

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Immune anticipation of mating in Drosophila : Turandot M promotes immunity against sexually transmitted fungal infections. / Zhong, Weihao; Mcclure, Colin; Evans, Cara; Mlynski, David; Immonen, Elina; Ritchie, Michael; Priest, N K.

In: Proceedings of the Royal Society B: Biological Sciences, Vol. 280, No. 1773, 20132018, 22.12.2013.

Research output: Contribution to journalArticle

Zhong, Weihao ; Mcclure, Colin ; Evans, Cara ; Mlynski, David ; Immonen, Elina ; Ritchie, Michael ; Priest, N K. / Immune anticipation of mating in Drosophila : Turandot M promotes immunity against sexually transmitted fungal infections. In: Proceedings of the Royal Society B: Biological Sciences. 2013 ; Vol. 280, No. 1773.
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