@inbook{5c2f5831f8da471dab051686912779dd,
title = "Imaging “Hot-Wired” clathrin-mediated endocytosis",
abstract = "Clathrin-mediated endocytosis (CME) occurs continuously at the plasma membrane of eukaryotic cells. However, when a vesicle forms and what cargo it contains are unpredictable. We recently developed a system to trigger CME on-demand. This means that we can control when endocytosis is triggered and the design means that the cargo that is internalized is predetermined. The method is called hot-wired CME because several steps and proteins are bypassed in our system. In this chapter, we describe in detail how to use the hot-wiring system to trigger endocytosis in human cell lines and how to image the vesicles that form using microscopy and finally, how to analyze those images.",
keywords = "Chemically induced dimerization, Clathrin, Endocytosis, Image analysis, Immunofluorescence, Live cell imaging",
author = "Wood, {Laura A.} and Royle, {Stephen J.}",
year = "2018",
doi = "10.1007/978-1-4939-8719-1_7",
language = "English",
isbn = "9781493987177",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "83--94",
booktitle = "Methods in Molecular Biology",
address = "USA United States",
}