Imaging drug delivery to skin with stimulated Raman scattering microscopy

Research output: Contribution to journalArticle

94 Citations (Scopus)

Abstract

Efficient drug delivery to the skin is essential for the treatment of major dermatologic diseases, such as eczema, psoriasis and acne. However, many compounds penetrate the skin barrier poorly and require optimized formulations to ensure their bioavailability. Here, stimulated Raman scattering (SRS) microscopy, a recently developed, label-free chemical imaging tool, is used to acquire high resolution images of multiple chemical components of a topical formulation as it penetrates into mammalian skin. This technique uniquely provides label-free, nondestructive, three-dimensional images with high spatiotemporal resolution. It reveals novel features of (trans)dermal drug delivery in the tissue environment: different rates of drug penetration via hair follicles as compared to the intercellular pathway across the stratum corneum are directly observed, and the precipitation of drug crystals on the skin surface is visualized after the percutaneous penetration of the cosolvent excipient in the formulation. The high speed three-dimensional imaging capability of SRS thus reveals features that cannot be seen with other techniques, providing both kinetic information and mechanistic insight into the (trans)dermal drug delivery process.
LanguageEnglish
Pages969-975
Number of pages7
JournalMolecular Pharmaceutics
Volume8
Issue number3
Early online date4 May 2010
DOIs
StatusPublished - 6 Jun 2011

Fingerprint

Raman Spectrum Analysis
Microscopy
Skin
Pharmaceutical Preparations
Three-Dimensional Imaging
Hair Follicle
Excipients
Eczema
Acne Vulgaris
Psoriasis
Cornea
Biological Availability

Keywords

  • dermatopharmacokinetics
  • stimulated Raman scattering microscopy
  • topical drug delivery
  • skin penetration pathways
  • skin

Cite this

Imaging drug delivery to skin with stimulated Raman scattering microscopy. / Saar, B G; Contreras-Rojas, Luis R; Xie, X S; Guy, Richard H.

In: Molecular Pharmaceutics, Vol. 8, No. 3, 06.06.2011, p. 969-975.

Research output: Contribution to journalArticle

@article{e6886adb45904d659b869031cd9caf34,
title = "Imaging drug delivery to skin with stimulated Raman scattering microscopy",
abstract = "Efficient drug delivery to the skin is essential for the treatment of major dermatologic diseases, such as eczema, psoriasis and acne. However, many compounds penetrate the skin barrier poorly and require optimized formulations to ensure their bioavailability. Here, stimulated Raman scattering (SRS) microscopy, a recently developed, label-free chemical imaging tool, is used to acquire high resolution images of multiple chemical components of a topical formulation as it penetrates into mammalian skin. This technique uniquely provides label-free, nondestructive, three-dimensional images with high spatiotemporal resolution. It reveals novel features of (trans)dermal drug delivery in the tissue environment: different rates of drug penetration via hair follicles as compared to the intercellular pathway across the stratum corneum are directly observed, and the precipitation of drug crystals on the skin surface is visualized after the percutaneous penetration of the cosolvent excipient in the formulation. The high speed three-dimensional imaging capability of SRS thus reveals features that cannot be seen with other techniques, providing both kinetic information and mechanistic insight into the (trans)dermal drug delivery process.",
keywords = "dermatopharmacokinetics, stimulated Raman scattering microscopy, topical drug delivery, skin penetration pathways, skin",
author = "Saar, {B G} and Contreras-Rojas, {Luis R} and Xie, {X S} and Guy, {Richard H}",
year = "2011",
month = "6",
day = "6",
doi = "10.1021/mp200122w",
language = "English",
volume = "8",
pages = "969--975",
journal = "Molecular Pharmaceutics",
issn = "1543-8384",
publisher = "American Chemical Society",
number = "3",

}

TY - JOUR

T1 - Imaging drug delivery to skin with stimulated Raman scattering microscopy

AU - Saar, B G

AU - Contreras-Rojas, Luis R

AU - Xie, X S

AU - Guy, Richard H

PY - 2011/6/6

Y1 - 2011/6/6

N2 - Efficient drug delivery to the skin is essential for the treatment of major dermatologic diseases, such as eczema, psoriasis and acne. However, many compounds penetrate the skin barrier poorly and require optimized formulations to ensure their bioavailability. Here, stimulated Raman scattering (SRS) microscopy, a recently developed, label-free chemical imaging tool, is used to acquire high resolution images of multiple chemical components of a topical formulation as it penetrates into mammalian skin. This technique uniquely provides label-free, nondestructive, three-dimensional images with high spatiotemporal resolution. It reveals novel features of (trans)dermal drug delivery in the tissue environment: different rates of drug penetration via hair follicles as compared to the intercellular pathway across the stratum corneum are directly observed, and the precipitation of drug crystals on the skin surface is visualized after the percutaneous penetration of the cosolvent excipient in the formulation. The high speed three-dimensional imaging capability of SRS thus reveals features that cannot be seen with other techniques, providing both kinetic information and mechanistic insight into the (trans)dermal drug delivery process.

AB - Efficient drug delivery to the skin is essential for the treatment of major dermatologic diseases, such as eczema, psoriasis and acne. However, many compounds penetrate the skin barrier poorly and require optimized formulations to ensure their bioavailability. Here, stimulated Raman scattering (SRS) microscopy, a recently developed, label-free chemical imaging tool, is used to acquire high resolution images of multiple chemical components of a topical formulation as it penetrates into mammalian skin. This technique uniquely provides label-free, nondestructive, three-dimensional images with high spatiotemporal resolution. It reveals novel features of (trans)dermal drug delivery in the tissue environment: different rates of drug penetration via hair follicles as compared to the intercellular pathway across the stratum corneum are directly observed, and the precipitation of drug crystals on the skin surface is visualized after the percutaneous penetration of the cosolvent excipient in the formulation. The high speed three-dimensional imaging capability of SRS thus reveals features that cannot be seen with other techniques, providing both kinetic information and mechanistic insight into the (trans)dermal drug delivery process.

KW - dermatopharmacokinetics

KW - stimulated Raman scattering microscopy

KW - topical drug delivery

KW - skin penetration pathways

KW - skin

UR - http://www.scopus.com/inward/record.url?scp=79958250060&partnerID=8YFLogxK

UR - http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3109166/

UR - http://dx.doi.org/10.1021/mp200122w

U2 - 10.1021/mp200122w

DO - 10.1021/mp200122w

M3 - Article

VL - 8

SP - 969

EP - 975

JO - Molecular Pharmaceutics

T2 - Molecular Pharmaceutics

JF - Molecular Pharmaceutics

SN - 1543-8384

IS - 3

ER -