Abstract
Background and Aim: Detection of early-stage pancreatic cancer (PaC) patients can decrease the mortality rate through surgical resection thanks to a screening approach of high-risk and asymptomatic individuals. Up to 80% of PaC patients are either hyperglycemic or diabetic at the time of diagnosis. Diabetes mellitus (DM) identification as an early manifestation of PaC may lead to detection of this malignancy at early and presymptomatic stages. Therefore, the current study aims to identify common molecular factors between DM and PaC to shed light on a potential screening for DM as a diagnostic approach for PaC. Methods: The study was divided into three steps including dataset selection and identification of key genes, quantitative real-time polymerase chain reaction (qRT-PCR) to validate these genes, and enrichment analysis of their target genes. Results: Using GEO2R analysis, conducted on microarray dataset (GSE15932), SPI1 and Yin Yang 1 (YY1) genes were found to be expressed in peripheral blood mononuclear cells of PaC, DM, and PaC + DM patients. Moreover, qRT-PCR results in validation of microarray data showed a significant increment of these two genes among all patient groups. Furthermore, enrichment analyses of SPI1 and YY1 target genes revealed that they are mainly expressed in hematopoietic cells and associated with immune responses as well as immune signaling pathways. Discussion: We speculate that this study on SPI1 and YY1 genes and their targets can result in a successful strategy to investigate diabetes as a screening step for better management of PaC screening using standard serologic tests.
Original language | English |
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Pages (from-to) | 286-294 |
Number of pages | 9 |
Journal | Biomedical and Biotechnology Research Journal |
Volume | 5 |
Issue number | 3 |
DOIs | |
Publication status | Published - 7 Sept 2021 |
Bibliographical note
Funding Information:This study was conducted in the Cellular and Molecular Biology Research Center and supported by the Biotechnology Department, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences (Tehran, Iran) with grant number 8701. We are grateful to Dr. A. Mohamadkhani for sample collecting, Dr. M. Moradi Chalashtari and A. Kouchaki for qRT-PCR technique consulting, and Dr. S. Bahrami and Dr. H. Rezaee for their critical suggestions regarding improving the manuscript.
Funding Information:
This paper was extracted from Sima Kalantari`s PhD thesis and supported by the School of advanced technologies in Medicine , Shahid Beheshti University of Medical Sciences, Tehran, Iran, through grant NO 8701.
Keywords
- Blood test
- diabetes mellitus
- pancreatic cancer
- screening
ASJC Scopus subject areas
- Biotechnology