Projects per year
We have recently developed a photolabeling method to identify GTP-loaded Rab proteins. The new biotinylated GTP analogue (Bio-ATB-GTP) binds to GTP-binding proteins and after a UV irradiation a covalent bond is formed between the protein and the photoreactive diazirine group on the photolabel. The tagged protein can then be isolated and detected using the classic biotin-streptavidin interaction. In this chapter, we describe the Bio-ATB-GTP photolabel and discuss the advantages of using this photolabeling approach to detect GTP-loaded Rab proteins compared to other existing methodologies. We also describe a step-by-step procedure for detecting the activated state of a Rab protein in primary rat adipocytes.
|Title of host publication||Glucose Transport|
|Subtitle of host publication||Methods and Protocols|
|Editors||Karin Lindkvist-Petersson, Jesper S. Hansen|
|Place of Publication||New York City, USA|
|Number of pages||14|
|Publication status||E-pub ahead of print - 8 Dec 2017|
|Name||Methods in Molecular Biology|
- Journal Article
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- 3 Finished
1/07/12 → 30/06/15
Project: Research council
1/07/09 → 30/06/11
Project: UK charity