Identification of glucose transporters in Aspergillus nidulans

Thaila Fernanda Dos Reis, João Filipe Menino, Vinícius Leite Pedro Bom, Neil Andrew Brown, Ana Cristina Colabardini, Marcela Savoldi, Maria Helena S Goldman, Fernando Rodrigues, Gustavo Henrique Goldman

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

To characterize the mechanisms involved in glucose transport, in the filamentous fungus Aspergillus nidulans, we have identified four glucose transporter encoding genes hxtB-E. We evaluated the ability of hxtB-E to functionally complement the Saccharomyces cerevisiae EBY.VW4000 strain that is unable to grow on glucose, fructose, mannose or galactose as single carbon source. In S. cerevisiae HxtB-E were targeted to the plasma membrane. The expression of HxtB, HxtC and HxtE was able to restore growth on glucose, fructose, mannose or galactose, indicating that these transporters accept multiple sugars as a substrate through an energy dependent process. A tenfold excess of unlabeled maltose, galactose, fructose, and mannose were able to inhibit glucose uptake to different levels (50 to 80%) in these s. cerevisiae complemented strains. Moreover, experiments with cyanide-mchlorophenylhydrazone (CCCP), strongly suggest that hxtB, -C, and -E mediate glucose transport via active proton symport. The A. nidulans ΔhxtB, ΔhxtC or ΔhxtE null mutants showed ∼2.5-fold reduction in the affinity for glucose, while ΔhxtB and -C also showed a 2-fold reduction in the capacity for glucose uptake. The ΔhxtD mutant had a 7.8- fold reduction in affinity, but a 3-fold increase in the capacity for glucose uptake. However, only the ΔhxtB mutant strain showed a detectable decreased rate of glucose consumption at low concentrations and an increased resistance to 2-deoxyglucose.

Original languageEnglish
Article numbere81412
JournalPLoS ONE
Volume8
Issue number11
DOIs
Publication statusPublished - 25 Nov 2013

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

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