Hypomethylated pollen bypasses the interploidy hybridization barrier in arabidopsis

Nicole Schatlowski, Philip Wolff, Juan Santos-González, Vera Schoft, Alexey Siretskiy, Rod Scott, Hisashi Tamaru, Claudia Köhler

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Plants of different ploidy levels are separated by a strong postzygotic hybridization barrier that is established in the endosperm. Deregulated parent-of-origin specific genes cause the response to interploidy hybridizations, revealing an epigenetic basis of this phenomenon. In this study, we present evidence that paternal hypomethylation can bypass the interploidy hybridization barrier by alleviating the requirement for the Polycomb Repressive Complex 2 (PRC2) in the endosperm. PRC2 epigenetically regulates gene expression by applying methylation marks on histone H3. Bypass of the barrier is mediated by suppressed expression of imprinted genes. We show that the hypomethylated pollen genome causes de novo CHG methylation directed to FIS-PRC2 target genes, suggesting that different epigenetic modifications can functionally substitute for each other. Our work presents a method for the generation of viable triploids, providing an impressive example of the potential of epigenome manipulations for plant breeding.

Original languageEnglish
Pages (from-to)3556-3568
Number of pages13
JournalPlant Cell
Volume26
Issue number9
DOIs
Publication statusPublished - 14 Sep 2014

Fingerprint

Polycomb Repressive Complex 2
Reproductive Isolation
Pollen
Arabidopsis
Endosperm
hybridization
pollen
Epigenomics
epigenetics
methylation
Methylation
endosperm
Gene Expression
Triploidy
gene expression
Ploidies
triploidy
plant breeding
ploidy
histones

Cite this

Schatlowski, N., Wolff, P., Santos-González, J., Schoft, V., Siretskiy, A., Scott, R., ... Köhler, C. (2014). Hypomethylated pollen bypasses the interploidy hybridization barrier in arabidopsis. Plant Cell, 26(9), 3556-3568. https://doi.org/10.1105/tpc.114.130120

Hypomethylated pollen bypasses the interploidy hybridization barrier in arabidopsis. / Schatlowski, Nicole; Wolff, Philip; Santos-González, Juan; Schoft, Vera; Siretskiy, Alexey; Scott, Rod; Tamaru, Hisashi; Köhler, Claudia.

In: Plant Cell, Vol. 26, No. 9, 14.09.2014, p. 3556-3568.

Research output: Contribution to journalArticle

Schatlowski, N, Wolff, P, Santos-González, J, Schoft, V, Siretskiy, A, Scott, R, Tamaru, H & Köhler, C 2014, 'Hypomethylated pollen bypasses the interploidy hybridization barrier in arabidopsis', Plant Cell, vol. 26, no. 9, pp. 3556-3568. https://doi.org/10.1105/tpc.114.130120
Schatlowski N, Wolff P, Santos-González J, Schoft V, Siretskiy A, Scott R et al. Hypomethylated pollen bypasses the interploidy hybridization barrier in arabidopsis. Plant Cell. 2014 Sep 14;26(9):3556-3568. https://doi.org/10.1105/tpc.114.130120
Schatlowski, Nicole ; Wolff, Philip ; Santos-González, Juan ; Schoft, Vera ; Siretskiy, Alexey ; Scott, Rod ; Tamaru, Hisashi ; Köhler, Claudia. / Hypomethylated pollen bypasses the interploidy hybridization barrier in arabidopsis. In: Plant Cell. 2014 ; Vol. 26, No. 9. pp. 3556-3568.
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