Hydrothermal conversion of one-photon-fluorescent poly-(4-vinylpyridine) into two-photon-fluorescent carbon nanodots

Katherine Lawrence, Fengjie Xia, Rory L Arrowsmith, Haobo Ge, Geoffrey W Nelson, John S Foord, Monica Felipe-Sotelo, Nick D M Evans, John M. Mitchels, Stephen E Flower, Stanley W Botchway, Daniel Wolverson, Gazi Aliev, Tony D James, Sofia I. Pascu, Frank Marken

Research output: Contribution to journalArticle

  • 16 Citations

Abstract

A novel two-photon-fluorescent N,O-heteroatom-rich carbon nanomaterial has been synthesized and characterized. The new carbon nanoparticles were produced by hydro-thermal conversion from a one-photon-fluorescent poly-(4-vinylpyridine) precursor (P4VP). The carbonized particles (cP4VP-dots) with non-uniform particle diameter (ranging from sub-6 nm to 20 nm with some aggregates up to 200 nm) exhibit strong fluorescent properties in different solvents and have also been investigated for applications in cell culture media. The cP4VP-dots retain their intrinsic fluorescence in a cellular environment and exhibit an average excited state lifetime of 2.0 ± 0.9 ns in cell. The cP4VP-dots enter HeLa cells and do not cause significant damage to outer cell membranes. They provide one-photon or two-photon fluorescent synthetic scaffolds for imaging applications and/or drug delivery.
LanguageEnglish
Pages11746–11752
JournalLangmuir
Volume30
Issue number39
Early online date12 Mar 2014
DOIs
StatusPublished - 7 Oct 2014

Fingerprint

Carbon
Photons
carbon
photons
culture media
Cell membranes
cells
Drug delivery
Cell culture
Excited states
Nanostructured materials
Scaffolds
Culture Media
delivery
drugs
Fluorescence
Nanoparticles
damage
Imaging techniques
life (durability)

Cite this

Hydrothermal conversion of one-photon-fluorescent poly-(4-vinylpyridine) into two-photon-fluorescent carbon nanodots. / Lawrence, Katherine; Xia, Fengjie; Arrowsmith, Rory L; Ge, Haobo; Nelson, Geoffrey W; Foord, John S; Felipe-Sotelo, Monica; Evans, Nick D M; Mitchels, John M.; Flower, Stephen E; Botchway, Stanley W; Wolverson, Daniel; Aliev, Gazi; James, Tony D; Pascu, Sofia I.; Marken, Frank.

In: Langmuir , Vol. 30, No. 39, 07.10.2014, p. 11746–11752.

Research output: Contribution to journalArticle

Lawrence, K, Xia, F, Arrowsmith, RL, Ge, H, Nelson, GW, Foord, JS, Felipe-Sotelo, M, Evans, NDM, Mitchels, JM, Flower, SE, Botchway, SW, Wolverson, D, Aliev, G, James, TD, Pascu, SI & Marken, F 2014, 'Hydrothermal conversion of one-photon-fluorescent poly-(4-vinylpyridine) into two-photon-fluorescent carbon nanodots' Langmuir , vol 30, no. 39, pp. 11746–11752. DOI: 10.1021/la404866s
Lawrence K, Xia F, Arrowsmith RL, Ge H, Nelson GW, Foord JS et al. Hydrothermal conversion of one-photon-fluorescent poly-(4-vinylpyridine) into two-photon-fluorescent carbon nanodots. Langmuir . 2014 Oct 7;30(39):11746–11752. Available from, DOI: 10.1021/la404866s
Lawrence, Katherine ; Xia, Fengjie ; Arrowsmith, Rory L ; Ge, Haobo ; Nelson, Geoffrey W ; Foord, John S ; Felipe-Sotelo, Monica ; Evans, Nick D M ; Mitchels, John M. ; Flower, Stephen E ; Botchway, Stanley W ; Wolverson, Daniel ; Aliev, Gazi ; James, Tony D ; Pascu, Sofia I. ; Marken, Frank. / Hydrothermal conversion of one-photon-fluorescent poly-(4-vinylpyridine) into two-photon-fluorescent carbon nanodots. In: Langmuir . 2014 ; Vol. 30, No. 39. pp. 11746–11752
@article{14b6845c55614d8dad02e5c31d39c3e1,
title = "Hydrothermal conversion of one-photon-fluorescent poly-(4-vinylpyridine) into two-photon-fluorescent carbon nanodots",
abstract = "A novel two-photon-fluorescent N,O-heteroatom-rich carbon nanomaterial has been synthesized and characterized. The new carbon nanoparticles were produced by hydro-thermal conversion from a one-photon-fluorescent poly-(4-vinylpyridine) precursor (P4VP). The carbonized particles (cP4VP-dots) with non-uniform particle diameter (ranging from sub-6 nm to 20 nm with some aggregates up to 200 nm) exhibit strong fluorescent properties in different solvents and have also been investigated for applications in cell culture media. The cP4VP-dots retain their intrinsic fluorescence in a cellular environment and exhibit an average excited state lifetime of 2.0 ± 0.9 ns in cell. The cP4VP-dots enter HeLa cells and do not cause significant damage to outer cell membranes. They provide one-photon or two-photon fluorescent synthetic scaffolds for imaging applications and/or drug delivery.",
author = "Katherine Lawrence and Fengjie Xia and Arrowsmith, {Rory L} and Haobo Ge and Nelson, {Geoffrey W} and Foord, {John S} and Monica Felipe-Sotelo and Evans, {Nick D M} and Mitchels, {John M.} and Flower, {Stephen E} and Botchway, {Stanley W} and Daniel Wolverson and Gazi Aliev and James, {Tony D} and Pascu, {Sofia I.} and Frank Marken",
year = "2014",
month = "10",
day = "7",
doi = "10.1021/la404866s",
language = "English",
volume = "30",
pages = "11746–11752",
journal = "Langmuir",
issn = "0743-7463",
publisher = "American Chemical Society",
number = "39",

}

TY - JOUR

T1 - Hydrothermal conversion of one-photon-fluorescent poly-(4-vinylpyridine) into two-photon-fluorescent carbon nanodots

AU - Lawrence,Katherine

AU - Xia,Fengjie

AU - Arrowsmith,Rory L

AU - Ge,Haobo

AU - Nelson,Geoffrey W

AU - Foord,John S

AU - Felipe-Sotelo,Monica

AU - Evans,Nick D M

AU - Mitchels,John M.

AU - Flower,Stephen E

AU - Botchway,Stanley W

AU - Wolverson,Daniel

AU - Aliev,Gazi

AU - James,Tony D

AU - Pascu,Sofia I.

AU - Marken,Frank

PY - 2014/10/7

Y1 - 2014/10/7

N2 - A novel two-photon-fluorescent N,O-heteroatom-rich carbon nanomaterial has been synthesized and characterized. The new carbon nanoparticles were produced by hydro-thermal conversion from a one-photon-fluorescent poly-(4-vinylpyridine) precursor (P4VP). The carbonized particles (cP4VP-dots) with non-uniform particle diameter (ranging from sub-6 nm to 20 nm with some aggregates up to 200 nm) exhibit strong fluorescent properties in different solvents and have also been investigated for applications in cell culture media. The cP4VP-dots retain their intrinsic fluorescence in a cellular environment and exhibit an average excited state lifetime of 2.0 ± 0.9 ns in cell. The cP4VP-dots enter HeLa cells and do not cause significant damage to outer cell membranes. They provide one-photon or two-photon fluorescent synthetic scaffolds for imaging applications and/or drug delivery.

AB - A novel two-photon-fluorescent N,O-heteroatom-rich carbon nanomaterial has been synthesized and characterized. The new carbon nanoparticles were produced by hydro-thermal conversion from a one-photon-fluorescent poly-(4-vinylpyridine) precursor (P4VP). The carbonized particles (cP4VP-dots) with non-uniform particle diameter (ranging from sub-6 nm to 20 nm with some aggregates up to 200 nm) exhibit strong fluorescent properties in different solvents and have also been investigated for applications in cell culture media. The cP4VP-dots retain their intrinsic fluorescence in a cellular environment and exhibit an average excited state lifetime of 2.0 ± 0.9 ns in cell. The cP4VP-dots enter HeLa cells and do not cause significant damage to outer cell membranes. They provide one-photon or two-photon fluorescent synthetic scaffolds for imaging applications and/or drug delivery.

UR - http://dx.doi.org/10.1021/la404866s

U2 - 10.1021/la404866s

DO - 10.1021/la404866s

M3 - Article

VL - 30

SP - 11746

EP - 11752

JO - Langmuir

T2 - Langmuir

JF - Langmuir

SN - 0743-7463

IS - 39

ER -