Hydrophilic carbon nanoparticle-laccase thin film electrode for mediatorless dioxygen reduction SECM activity mapping and application in zinc-dioxygen battery

K Szot, W Nogala, J Niedziolka-Jonsson, M Jonsson-Niedziolka, Frank Marken, J Rogalski, C N Kirchner, G Wittstock, M Opallo

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61 Citations (Scopus)

Abstract

Laccase from Cerrena unicolor was adsorbed on hydrophilic carbon nanoparticles (diameter = ca. 7.8 nm) modified with phenyl sulfonate groups and immobilized on an ITO electrode surface in a sol-gel processed silicate film. As shown by scanning electron and atomic force microscopies, the nanoparticles are evenly distributed on the electrode surface forming small aggregates of tens of nanometers in size. The mediator-free electrode exhibits significant and pH-dependent electrocatalytic activity towards dioxygen reduction. The maximum catalytic current density (95 mu A cm(-2)) is obtained at pH 4.8 corresponding to maximum activity of the enzyme. Under these conditions dioxygen electroreduction commences at 0.575 V vs. Ag vertical bar AgClsat, a value close to the formal potential of the T1 redox centre of the laccase. The scanning electrochemical microscopy images obtained in redox competition mode exploiting mediatorless electrocatalysis show that the laccase is evenly distributed in the composite him. The obtained electrode was applied as biocathode in a zinc-dioxygen battery operating in 0.1 M McIlvaine buffer (pH 4.8). It provides 1.48V at open circuit and a maximum power density 17.4 mu W cm(-2) at 0.7 V.
Original languageEnglish
Pages (from-to)4620-4625
Number of pages6
JournalElectrochimica Acta
Volume54
Issue number20
DOIs
Publication statusPublished - 2009

Keywords

  • Laccase
  • Direct electron transfer
  • Dioxygen reduction
  • Scanning electrochemical microscopy (SECM)
  • Carbon nanoparticles

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