We report on a novel electroanalytical system for hydrogen peroxide (H O) detection in humidity or droplets of aerosol, formed by air bubbling through a washing chamber; the resulting flow mimics the exhaled human breath. The system is based on a planar three-electrode structure (with a Prussian Blue based HO transducer modified working electrode) bridged by a solid salt-saturated filament material (filter paper, cotton textile). Respective to the hydrogen peroxide content in the washing valve, the response of the aerosol-sensing system is linear in the concentration range of 0.1-10 μM, which overlaps the generally accepted H O content in exhaled breath condensate (EBC), with the sensitivity of 8 A M cm. The response to the upper limit of the calibration range is stable for more than 50 injection cycles recorded within 3 days. Both the stability and the suitable calibration range allow one to consider the reported aerosol-sensing system as a prototype for a simple (avoiding intermediate EBC collection) noninvasive diagnostic tool for pulmonary patients.
Komkova, M. A., Karyakina, E. E., Marken, F., & Karyakin, A. A. (2013). Hydrogen peroxide detection in wet air with a Prussian Blue based solid salt bridged three electrode system. Analytical Chemistry, 85(5), 2574-2577. https://doi.org/10.1021/ac303761h