Hybrid Hierarchical Heterostructures of Nanoceramic Phosphors as Imaging Agents for Multiplexing and Living Cancer Cells Translocation

David Gonzalez Calatayud, Teresa Jardiel, Mara S. Bernardo, Vincenzo Mirabello, Haobo Ge, Rory Arrowsmith, Fernando Cortezon-Tamarit, Lorena Alcaraz, Josefa Isasi, Pablo Arevalo, Amador C. Cabellero, Sofia Pascu, Marco Peiteado

Research output: Contribution to journalArticlepeer-review

1 Citation (SciVal)

Abstract

Existing fluorescent labels used in life sciences are based on organic compounds with limited lifetime or on quantum dots which are either expensive or toxic and have low kinetic stability in biological environments. To address these challenges, luminescent nanomaterials have been conceived as hierarchical, core–shell structures with spherical morphology and highly controlled dimensions. These tailor-made nanophosphors incorporate Ln:YVO4 nanoparticles (Ln = Eu(III) and Er(III)) as 50 nm cores and display intense and narrow emission maxima centered at ∼565 nm. These cores can be encapsulated in silica shells with highly controlled dimensions as well as functionalized with chitosan or PEG5000 to reduce nonspecific interactions with biomolecules in living cells. Confocal fluorescence microscopy in living prostate cancer cells confirmed the potential of these platforms to overcome the disadvantages of commercial fluorophores and their feasibility as labels for multiplexing, biosensing, and imaging in life science assays.
Original languageEnglish
Pages (from-to)4105–4118
JournalACS Applied Biomaterials
Volume4
Issue number5
Early online date10 Mar 2021
DOIs
Publication statusPublished - 17 May 2021

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