Human serum albumin-based supramolecular host-guest boronate probe for enhanced peroxynitrite sensing

He Tian, Chen Guo, Xi Le Hu, Jing Bo Wang, Yi Zang, Tony D. James, Jia Li, Xiao Peng He

Research output: Contribution to journalArticlepeer-review

Abstract

Peroxynitrite (ONOO) is an important oxygen/nitrogen reactive species implicated in a number of physiological and pathological processes. However, due to the complexity of the cellular micro-environment, the sensitive and accurate detection of ONOO remains a challenging task. Here, we developed a long-wavelength fluorescent probe based on the conjugation between a TCF scaffold and phenylboronate; the resulting conjugate is capable of supramolecular host-guest assembly with human serum albumin (HSA) for the fluorogenic sensing of ONOO. The probe exhibited an enhanced fluorescence over a low concentration range of ONOO (0-9.6 μM), whist the fluorescence was quenched when the concentration of ONOO exceeded 9.6 μM. In addition, when human serum albumin (HSA) was added, the initial fluorescence of the probe was significantly enhanced, which enabled the more sensitive detection of low-concentrations of ONOO in aqueous buffer solution and in cells. The molecular structure of the supramolecular host-guest ensemble was determined using small-angle X-ray scattering.

Original languageEnglish
Pages (from-to)4661-4666
Number of pages6
JournalOrganic and Biomolecular Chemistry
Volume21
Issue number22
Early online date22 May 2023
DOIs
Publication statusPublished - 14 Jun 2023

Bibliographical note

Funding Information:
The authors thank the National Natural Science Foundation of China (NSFC) (No. 92253306 and 82130099), the Shanghai Municipal Science and Technology Major Project (No. 2018SHZDZX03), the Fundamental Research Funds for the Central Universities (222201717003), the Programme of Introducing Talents of Discipline to Universities (B16017) and Open Funding Project of the State Key Laboratory of Bioreactor Engineering of East China University of Science and Technology for financial support. The Research Center of Analysis and Test of East China University of Science and Technology is gratefully acknowledged for assistance in analytical experiments. TDJ wishes to thank the University of Bath and the Open Research Fund of the School of Chemistry and Chemical Engineering, Henan Normal University (2020ZD01) for support.

ASJC Scopus subject areas

  • Biochemistry
  • Physical and Theoretical Chemistry
  • Organic Chemistry

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