Human fibroblast culture on a crosslinked dermal porcine collagen matrix

Marcus Jarman-Smith, Tulin Bodamyali, Cliff Stevens, John A. Howell, Michael Horrocks, Julian B Chaudhuri

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The use of a novel porcine-derived collagen biomaterial as a dermal tissue engineering matrix was examd. The matrix is derived from porcine dermis, and is processed to retain the native collagen (Type 1) and elastin structure. Human primary fibroblasts were cultured on the matrix to examine its potential for creating a dermal replacement. Attachment of fibroblasts on the collagen was compared to tissue culture plastic and PET membranes. Cell proliferation was assessed using the MTT assay and DAPI staining. For seeding densities of 5*104 and 1*105 cells cm-2, PET and plastic demonstrated >95% attachment of seeded nos. after 3 h. The collagen matrix reached levels >80% after 3-4 h with no influence of the seeding d. Matrix samples with perforating pores of 40 micro m diam. were also studied. After 216 h culture in static culture, with media replacement every 3 days, the final cell nos. reached 2.1*105 (perforated) and 2.0*105 cells cm-2 (unperforated). In comparison fibroblast culture in a perfusion bioreactor, with continuous media replacement, reached 2.3*105 (unperforated) and 2.5*105 cells cm-2 (perforated) after 216 h. [on SciFinder (R)]
Original languageEnglish
Pages (from-to)217-222
Number of pages6
JournalBiochemical Engineering Journal
Volume20
Issue number2-3
Publication statusPublished - 2004

Fingerprint

Fibroblasts
Cell culture
Collagen
Swine
Skin
Plastics
Perforating
Tissue culture
Elastin
Cell proliferation
Biocompatible Materials
Collagen Type I
Bioreactors
Tissue engineering
Assays
Tissue Engineering
Dermis
Biomaterials
Membranes
Culture Media

Keywords

  • BUU (Biological use
  • fibroblast crosslinked skin collagen matrix
  • Animal tissue culture
  • Human
  • unclassified)
  • BIOL (Biological study)
  • PRP (Properties)
  • BIOL (Biological study) (human fibroblast culture on crosslinked dermal porcine collagen matrix)
  • human fibroblast culture on crosslinked dermal porcine collagen matrix)
  • USES (Uses) (type I
  • BSU (Biological study
  • Elastins Role
  • Skin (engineering
  • Fibroblast
  • Sus scrofa domestica (human fibroblast culture on crosslinked dermal porcine collagen matrix)
  • Cell proliferation
  • Collagens Role

Cite this

Jarman-Smith, M., Bodamyali, T., Stevens, C., Howell, J. A., Horrocks, M., & Chaudhuri, J. B. (2004). Human fibroblast culture on a crosslinked dermal porcine collagen matrix. Biochemical Engineering Journal, 20(2-3), 217-222.

Human fibroblast culture on a crosslinked dermal porcine collagen matrix. / Jarman-Smith, Marcus; Bodamyali, Tulin; Stevens, Cliff; Howell, John A.; Horrocks, Michael; Chaudhuri, Julian B.

In: Biochemical Engineering Journal, Vol. 20, No. 2-3, 2004, p. 217-222.

Research output: Contribution to journalArticle

Jarman-Smith, M, Bodamyali, T, Stevens, C, Howell, JA, Horrocks, M & Chaudhuri, JB 2004, 'Human fibroblast culture on a crosslinked dermal porcine collagen matrix', Biochemical Engineering Journal, vol. 20, no. 2-3, pp. 217-222.
Jarman-Smith M, Bodamyali T, Stevens C, Howell JA, Horrocks M, Chaudhuri JB. Human fibroblast culture on a crosslinked dermal porcine collagen matrix. Biochemical Engineering Journal. 2004;20(2-3):217-222.
Jarman-Smith, Marcus ; Bodamyali, Tulin ; Stevens, Cliff ; Howell, John A. ; Horrocks, Michael ; Chaudhuri, Julian B. / Human fibroblast culture on a crosslinked dermal porcine collagen matrix. In: Biochemical Engineering Journal. 2004 ; Vol. 20, No. 2-3. pp. 217-222.
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AB - The use of a novel porcine-derived collagen biomaterial as a dermal tissue engineering matrix was examd. The matrix is derived from porcine dermis, and is processed to retain the native collagen (Type 1) and elastin structure. Human primary fibroblasts were cultured on the matrix to examine its potential for creating a dermal replacement. Attachment of fibroblasts on the collagen was compared to tissue culture plastic and PET membranes. Cell proliferation was assessed using the MTT assay and DAPI staining. For seeding densities of 5*104 and 1*105 cells cm-2, PET and plastic demonstrated >95% attachment of seeded nos. after 3 h. The collagen matrix reached levels >80% after 3-4 h with no influence of the seeding d. Matrix samples with perforating pores of 40 micro m diam. were also studied. After 216 h culture in static culture, with media replacement every 3 days, the final cell nos. reached 2.1*105 (perforated) and 2.0*105 cells cm-2 (unperforated). In comparison fibroblast culture in a perfusion bioreactor, with continuous media replacement, reached 2.3*105 (unperforated) and 2.5*105 cells cm-2 (perforated) after 216 h. [on SciFinder (R)]

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