How diverse is the genus Wolbachia? Multiple-gene sequencing reveals a putatively new Wolbachia supergroup recovered from spider mites (Acari: Tetranychidae)

Vera I D Ros, V M Fleming, Edward J Feil, Johannes A J Breeuwer

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Abstract

At least 20% of all arthropods and some nematode species are infected with intracellular bacteria of the genus Wolbachia. This highly diverse genus has been subdivided into eight "supergroups" (A to H) on the basis of nucleotide sequence data. Here, we report the discovery of a new Wolbachia supergroup recovered from the spider mite species Bryobia species V (Acari: Tetranychidae), based on the sequences of three protein-coding genes (ftsZ, gltA, and groEL) and the 16S rRNA gene. Other tetranychid mites possess supergroup B Wolbachia strains. The discovery of another Wolbachia supergroup expands the known diversity of Wolbachia and emphasizes the high variability of the genus. Our data also clarify the existing supergroup structure and highlight the use of multiple gene sequences for robust phylogenetic analysis. In addition to previous reports of recombination between the arthropod-infecting supergroups A and B, we provide evidence for recombination between the nematode-infecting supergroups C and D. Robust delineation of supergroups is essential for understanding the origin and spread of this common reproductive parasite and for unraveling mechanisms of host adaptation and manipulation across a wide range of hosts.
Original languageEnglish
Pages (from-to)1036-1043
Number of pages8
JournalApplied and Environmental Microbiology
Volume75
Issue number4
DOIs
Publication statusPublished - Feb 2009

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Tetranychidae
Wolbachia
mite
spider
Acari
arthropod
recombination
nematode
gene
Arthropods
Genes
genes
Genetic Recombination
arthropods
Bryobia
Nematoda
parasite
nucleotide sequences
Mites
Host Specificity

Keywords

  • Genes
  • Nucleic acids

Cite this

How diverse is the genus Wolbachia? Multiple-gene sequencing reveals a putatively new Wolbachia supergroup recovered from spider mites (Acari: Tetranychidae). / Ros, Vera I D; Fleming, V M; Feil, Edward J; Breeuwer, Johannes A J.

In: Applied and Environmental Microbiology, Vol. 75, No. 4, 02.2009, p. 1036-1043.

Research output: Contribution to journalArticle

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