Host-Biomaterial Interactions in Zebrafish

Claire E. Witherel, David Gurevich, John D. Collin, Paul Martin, Kara L. Spiller

Research output: Contribution to journalArticlepeer-review

15 Citations (SciVal)


Host-biomaterial interactions are critical determinants of the success or failure of an implant. However, detailed understanding of this process is limited due to a lack of dynamic tools for in vivo analyses. Here we characterize host-biomaterial interactions in zebrafish (Danio rerio), which are optically translucent and genetically tractable. Histological and immunohistochemical analyses following polypropylene suture implantation into adult zebrafish showed prolonged elevation of immune cell recruitment and collagen deposition, resembling a foreign body response. Live in vivo analysis showed that adsorption of the immunomodulatory cytokine interleukin-10 to a polystyrene microparticle, microinjected into transgenic larval zebrafish, inhibited neutrophil recruitment after 24 h compared to control microparticles, with no change in macrophage recruitment. This study illustrates that zebrafish are useful to investigate host-biomaterial interactions and have potential for high-throughput analysis of novel immunomodulatory biomaterials.

Original languageEnglish
Pages (from-to)1233-1240
Number of pages8
JournalACS Biomaterials Science and Engineering
Issue number4
Early online date14 Apr 2017
Publication statusPublished - 9 Apr 2018

Bibliographical note

Funding Information:
C.E.W. acknowledges the generous support of the Whitaker International Fellowship. Additionally, the authors gratefully acknowledge the Wolfson Bioimaging Facility for their imaging support and specialized training. Zebrafish work in the Martin lab is funded by a Wellcome Trust Investigator Award WT097791/Z/11/Z. Work in the Spiller lab is funded by NHLBI R01 HL130037.

Publisher Copyright:
© 2017 American Chemical Society.

Copyright 2018 Elsevier B.V., All rights reserved.


  • biomaterial
  • immunomodulatory
  • inflammation
  • interleukin-10
  • zebrafish

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering


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