Host-Biomaterial Interactions in Zebrafish

Claire E. Witherel; David Gurevich; John D. Collin; Paul Martin; Kara L. Spiller

doi:10.1021/acsbiomaterials.6b00760

Host-Biomaterial Interactions in Zebrafish

Claire E. Witherel, David Gurevich, John D. Collin, Paul Martin, Kara L. Spiller

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

17 Citations (SciVal)

Abstract

Host-biomaterial interactions are critical determinants of the success or failure of an implant. However, detailed understanding of this process is limited due to a lack of dynamic tools for in vivo analyses. Here we characterize host-biomaterial interactions in zebrafish (Danio rerio), which are optically translucent and genetically tractable. Histological and immunohistochemical analyses following polypropylene suture implantation into adult zebrafish showed prolonged elevation of immune cell recruitment and collagen deposition, resembling a foreign body response. Live in vivo analysis showed that adsorption of the immunomodulatory cytokine interleukin-10 to a polystyrene microparticle, microinjected into transgenic larval zebrafish, inhibited neutrophil recruitment after 24 h compared to control microparticles, with no change in macrophage recruitment. This study illustrates that zebrafish are useful to investigate host-biomaterial interactions and have potential for high-throughput analysis of novel immunomodulatory biomaterials.

Original language	English
Pages (from-to)	1233-1240
Number of pages	8
Journal	ACS Biomaterials Science and Engineering
Volume	4
Issue number	4
Early online date	14 Apr 2017
DOIs	https://doi.org/10.1021/acsbiomaterials.6b00760
Publication status	Published - 9 Apr 2018

Bibliographical note

Funding Information:
C.E.W. acknowledges the generous support of the Whitaker International Fellowship. Additionally, the authors gratefully acknowledge the Wolfson Bioimaging Facility for their imaging support and specialized training. Zebrafish work in the Martin lab is funded by a Wellcome Trust Investigator Award WT097791/Z/11/Z. Work in the Spiller lab is funded by NHLBI R01 HL130037.

Publisher Copyright:
© 2017 American Chemical Society.

Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.

Funding

C.E.W. acknowledges the generous support of the Whitaker International Fellowship. Additionally, the authors gratefully acknowledge the Wolfson Bioimaging Facility for their imaging support and specialized training. Zebrafish work in the Martin lab is funded by a Wellcome Trust Investigator Award WT097791/Z/11/Z. Work in the Spiller lab is funded by NHLBI R01 HL130037.

Keywords

biomaterial
immunomodulatory
inflammation
interleukin-10
zebrafish

ASJC Scopus subject areas

Biomaterials
Biomedical Engineering

Access to Document

10.1021/acsbiomaterials.6b00760

https://research-information.bris.ac.uk/en/publications/host-biomaterial-interactions-in-zebrafish

Cite this

@article{ad4234b64cef48d48ac3ebc0ffac1404,

title = "Host-Biomaterial Interactions in Zebrafish",

abstract = "Host-biomaterial interactions are critical determinants of the success or failure of an implant. However, detailed understanding of this process is limited due to a lack of dynamic tools for in vivo analyses. Here we characterize host-biomaterial interactions in zebrafish (Danio rerio), which are optically translucent and genetically tractable. Histological and immunohistochemical analyses following polypropylene suture implantation into adult zebrafish showed prolonged elevation of immune cell recruitment and collagen deposition, resembling a foreign body response. Live in vivo analysis showed that adsorption of the immunomodulatory cytokine interleukin-10 to a polystyrene microparticle, microinjected into transgenic larval zebrafish, inhibited neutrophil recruitment after 24 h compared to control microparticles, with no change in macrophage recruitment. This study illustrates that zebrafish are useful to investigate host-biomaterial interactions and have potential for high-throughput analysis of novel immunomodulatory biomaterials.",

keywords = "biomaterial, immunomodulatory, inflammation, interleukin-10, zebrafish",

author = "Witherel, {Claire E.} and David Gurevich and Collin, {John D.} and Paul Martin and Spiller, {Kara L.}",

note = "Funding Information: C.E.W. acknowledges the generous support of the Whitaker International Fellowship. Additionally, the authors gratefully acknowledge the Wolfson Bioimaging Facility for their imaging support and specialized training. Zebrafish work in the Martin lab is funded by a Wellcome Trust Investigator Award WT097791/Z/11/Z. Work in the Spiller lab is funded by NHLBI R01 HL130037. Publisher Copyright: {\textcopyright} 2017 American Chemical Society. Copyright: Copyright 2018 Elsevier B.V., All rights reserved.",

year = "2018",

month = apr,

day = "9",

doi = "10.1021/acsbiomaterials.6b00760",

language = "English",

volume = "4",

pages = "1233--1240",

journal = "ACS Biomaterials Science and Engineering",

issn = "2373-9878",

publisher = "American Chemical Society",

number = "4",

}

TY - JOUR

T1 - Host-Biomaterial Interactions in Zebrafish

AU - Witherel, Claire E.

AU - Gurevich, David

AU - Collin, John D.

AU - Martin, Paul

AU - Spiller, Kara L.

N1 - Funding Information: C.E.W. acknowledges the generous support of the Whitaker International Fellowship. Additionally, the authors gratefully acknowledge the Wolfson Bioimaging Facility for their imaging support and specialized training. Zebrafish work in the Martin lab is funded by a Wellcome Trust Investigator Award WT097791/Z/11/Z. Work in the Spiller lab is funded by NHLBI R01 HL130037. Publisher Copyright: © 2017 American Chemical Society. Copyright: Copyright 2018 Elsevier B.V., All rights reserved.

PY - 2018/4/9

Y1 - 2018/4/9

N2 - Host-biomaterial interactions are critical determinants of the success or failure of an implant. However, detailed understanding of this process is limited due to a lack of dynamic tools for in vivo analyses. Here we characterize host-biomaterial interactions in zebrafish (Danio rerio), which are optically translucent and genetically tractable. Histological and immunohistochemical analyses following polypropylene suture implantation into adult zebrafish showed prolonged elevation of immune cell recruitment and collagen deposition, resembling a foreign body response. Live in vivo analysis showed that adsorption of the immunomodulatory cytokine interleukin-10 to a polystyrene microparticle, microinjected into transgenic larval zebrafish, inhibited neutrophil recruitment after 24 h compared to control microparticles, with no change in macrophage recruitment. This study illustrates that zebrafish are useful to investigate host-biomaterial interactions and have potential for high-throughput analysis of novel immunomodulatory biomaterials.

AB - Host-biomaterial interactions are critical determinants of the success or failure of an implant. However, detailed understanding of this process is limited due to a lack of dynamic tools for in vivo analyses. Here we characterize host-biomaterial interactions in zebrafish (Danio rerio), which are optically translucent and genetically tractable. Histological and immunohistochemical analyses following polypropylene suture implantation into adult zebrafish showed prolonged elevation of immune cell recruitment and collagen deposition, resembling a foreign body response. Live in vivo analysis showed that adsorption of the immunomodulatory cytokine interleukin-10 to a polystyrene microparticle, microinjected into transgenic larval zebrafish, inhibited neutrophil recruitment after 24 h compared to control microparticles, with no change in macrophage recruitment. This study illustrates that zebrafish are useful to investigate host-biomaterial interactions and have potential for high-throughput analysis of novel immunomodulatory biomaterials.

KW - biomaterial

KW - immunomodulatory

KW - inflammation

KW - interleukin-10

KW - zebrafish

UR - http://www.scopus.com/inward/record.url?scp=85045205067&partnerID=8YFLogxK

U2 - 10.1021/acsbiomaterials.6b00760

DO - 10.1021/acsbiomaterials.6b00760

M3 - Article

AN - SCOPUS:85045205067

SN - 2373-9878

VL - 4

SP - 1233

EP - 1240

JO - ACS Biomaterials Science and Engineering

JF - ACS Biomaterials Science and Engineering

IS - 4

ER -

Host-Biomaterial Interactions in Zebrafish

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this