Holographic aberration correction: Optimising the stiffness of an optical trap deep in the sample

M. Dienerowitz, G. Gibson, R. Bowman, M. Padgett

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

We investigate the effects of 1st order spherical aberration and defocus upon the stiffness of an optical trap tens of μm into the sample. We control both these aberrations with a spatial light modulator. The key to maintain optimum trap stiffness over a range of depths is a specific non-trivial combination of defocus and axial objective position. This optimisation increases the trap stiffness by up to a factor of 3 and allows trapping of 1μm polystyrene beads up to 50μm deep in the sample.
Original languageEnglish
Pages (from-to)24589-24595
Number of pages7
JournalOptics Express
Volume19
Issue number24
DOIs
Publication statusPublished - 2011

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