High-throughput production of silk fibroin-based electrospun fibers as biomaterial for skin tissue engineering applications

Antonios Keirouz; Mariia Zakharova; Jaehoon Kwon; Colin Robert; Vasileios Koutsos; Anthony Callanan; Xianfeng Chen; Giuseppino Fortunato; Norbert Radacsi

doi:10.1016/j.msec.2020.110939

High-throughput production of silk fibroin-based electrospun fibers as biomaterial for skin tissue engineering applications

Antonios Keirouz, Mariia Zakharova, Jaehoon Kwon, Colin Robert, Vasileios Koutsos, Anthony Callanan, Xianfeng Chen, Giuseppino Fortunato, Norbert Radacsi

Department of Chemical Engineering

Research output: Contribution to journal › Article › peer-review

63 Citations (SciVal)

Abstract

In this work, a nozzle-free electrospinning device was built to obtain high-throughput production of silk fibroin-based biocompatible composite fibers with tunable wettability. Synthetic biomaterials tend to present suboptimal cell growth and proliferation, with many studies linking this phenomenon to the hydrophobicity of such surfaces. In this study, electrospun mats consisting of Poly(caprolactone) blended with variant forms of Poly(glycerol sebacate) (PGS) and regenerated silk fibroin were fabricated. The main aim of this work was the development of fiber mats with tunable hydrophobicity/hydrophilicity properties depending on the esterification degree and concentration of PGS. A variation of the conventional protocol used for the extraction of silk fibroin from Bombyx mori cocoons was employed, achieving significantly increased yields of the protein, in a third of the time required via the conventional extraction protocol. By altering the surface properties of the electrospun membranes, the trinary composite biomaterial presented good in vitro fibroblast attachment behavior and optimal growth, indicating the potential of such constructs towards the development of an artificial skin-like platform that can aid wound healing and skin regeneration.

Original language	English
Article number	110939
Journal	Materials Science and Engineering C
Volume	112
Early online date	8 Apr 2020
DOIs	https://doi.org/10.1016/j.msec.2020.110939
Publication status	Published - 31 Jul 2020

Bibliographical note

Funding Information:
The authors would like to thank Dr. Junxi Wu from the Centre for Cardiovascular Science of The University of Edinburgh for donating the cell-line used in this study. Dr. Coinneach Dover, Prof Khellil Sefiane, and Dr. Mike Davidson for sharing their expertise and allowing us access and training to the water contact angle instrument and FTIR equipment, respectively. Furthermore, we would like to recognize the Castansa Trust for their donation of the SEM (JEOL JSM-IT100) to the Radacsi research group. We would also like to thank Fergus Dingwall and Wesley Shao for their appreciated laboratory assistance. We acknowledge the use of the Cryo FIB/SEM bought with the EPSRC grant EP/P030564/1 and Thomas Glen for help with image acquisition.

Publisher Copyright:
© 2020 Elsevier B.V.

Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.

Keywords

Nozzle-free electrospinning
Poly(caprolactone)
Poly(glycerol sebacate)
Silk fibroin
wound healing

ASJC Scopus subject areas

General Materials Science
Condensed Matter Physics
Mechanics of Materials
Mechanical Engineering

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10.1016/j.msec.2020.110939

https://www.research.ed.ac.uk/en/publications/high-throughput-production-of-silk-fibroin-based-electrospun-fibe

Cite this

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title = "High-throughput production of silk fibroin-based electrospun fibers as biomaterial for skin tissue engineering applications",

abstract = "In this work, a nozzle-free electrospinning device was built to obtain high-throughput production of silk fibroin-based biocompatible composite fibers with tunable wettability. Synthetic biomaterials tend to present suboptimal cell growth and proliferation, with many studies linking this phenomenon to the hydrophobicity of such surfaces. In this study, electrospun mats consisting of Poly(caprolactone) blended with variant forms of Poly(glycerol sebacate) (PGS) and regenerated silk fibroin were fabricated. The main aim of this work was the development of fiber mats with tunable hydrophobicity/hydrophilicity properties depending on the esterification degree and concentration of PGS. A variation of the conventional protocol used for the extraction of silk fibroin from Bombyx mori cocoons was employed, achieving significantly increased yields of the protein, in a third of the time required via the conventional extraction protocol. By altering the surface properties of the electrospun membranes, the trinary composite biomaterial presented good in vitro fibroblast attachment behavior and optimal growth, indicating the potential of such constructs towards the development of an artificial skin-like platform that can aid wound healing and skin regeneration.",

keywords = "Nozzle-free electrospinning, Poly(caprolactone), Poly(glycerol sebacate), Silk fibroin, wound healing",

author = "Antonios Keirouz and Mariia Zakharova and Jaehoon Kwon and Colin Robert and Vasileios Koutsos and Anthony Callanan and Xianfeng Chen and Giuseppino Fortunato and Norbert Radacsi",

note = "Funding Information: The authors would like to thank Dr. Junxi Wu from the Centre for Cardiovascular Science of The University of Edinburgh for donating the cell-line used in this study. Dr. Coinneach Dover, Prof Khellil Sefiane, and Dr. Mike Davidson for sharing their expertise and allowing us access and training to the water contact angle instrument and FTIR equipment, respectively. Furthermore, we would like to recognize the Castansa Trust for their donation of the SEM (JEOL JSM-IT100) to the Radacsi research group. We would also like to thank Fergus Dingwall and Wesley Shao for their appreciated laboratory assistance. We acknowledge the use of the Cryo FIB/SEM bought with the EPSRC grant EP/P030564/1 and Thomas Glen for help with image acquisition. Publisher Copyright: {\textcopyright} 2020 Elsevier B.V. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",

year = "2020",

month = jul,

day = "31",

doi = "10.1016/j.msec.2020.110939",

language = "English",

volume = "112",

journal = "Materials Science and Engineering C",

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AU - Keirouz, Antonios

AU - Zakharova, Mariia

AU - Kwon, Jaehoon

AU - Robert, Colin

AU - Koutsos, Vasileios

AU - Callanan, Anthony

AU - Chen, Xianfeng

AU - Fortunato, Giuseppino

AU - Radacsi, Norbert

N1 - Funding Information: The authors would like to thank Dr. Junxi Wu from the Centre for Cardiovascular Science of The University of Edinburgh for donating the cell-line used in this study. Dr. Coinneach Dover, Prof Khellil Sefiane, and Dr. Mike Davidson for sharing their expertise and allowing us access and training to the water contact angle instrument and FTIR equipment, respectively. Furthermore, we would like to recognize the Castansa Trust for their donation of the SEM (JEOL JSM-IT100) to the Radacsi research group. We would also like to thank Fergus Dingwall and Wesley Shao for their appreciated laboratory assistance. We acknowledge the use of the Cryo FIB/SEM bought with the EPSRC grant EP/P030564/1 and Thomas Glen for help with image acquisition. Publisher Copyright: © 2020 Elsevier B.V. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.

PY - 2020/7/31

Y1 - 2020/7/31

N2 - In this work, a nozzle-free electrospinning device was built to obtain high-throughput production of silk fibroin-based biocompatible composite fibers with tunable wettability. Synthetic biomaterials tend to present suboptimal cell growth and proliferation, with many studies linking this phenomenon to the hydrophobicity of such surfaces. In this study, electrospun mats consisting of Poly(caprolactone) blended with variant forms of Poly(glycerol sebacate) (PGS) and regenerated silk fibroin were fabricated. The main aim of this work was the development of fiber mats with tunable hydrophobicity/hydrophilicity properties depending on the esterification degree and concentration of PGS. A variation of the conventional protocol used for the extraction of silk fibroin from Bombyx mori cocoons was employed, achieving significantly increased yields of the protein, in a third of the time required via the conventional extraction protocol. By altering the surface properties of the electrospun membranes, the trinary composite biomaterial presented good in vitro fibroblast attachment behavior and optimal growth, indicating the potential of such constructs towards the development of an artificial skin-like platform that can aid wound healing and skin regeneration.

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KW - Poly(glycerol sebacate)

KW - Silk fibroin

KW - wound healing

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SN - 0928-4931

VL - 112

JO - Materials Science and Engineering C

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M1 - 110939

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High-throughput production of silk fibroin-based electrospun fibers as biomaterial for skin tissue engineering applications

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

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