High rates of recombination in otitis media isolates of non-typeable Haemophilus influenzae

Alison J. Cody; Dawn Field; Edward J. Feil; Suzanna Stringer; Mary E. Deadman; Anthony G. Tsolaki; Brett Gratz; Valérie Bouchet; Richard Goldstein; Derek W. Hood; E. Richard Moxon

doi:10.1016/S1567-1348(02)00152-1

High rates of recombination in otitis media isolates of non-typeable Haemophilus influenzae

Alison J. Cody
, Dawn Field
, Edward J. Feil
, Suzanna Stringer
, Mary E. Deadman
, Anthony G. Tsolaki
, Brett Gratz
, Valérie Bouchet
, Richard Goldstein
, Derek W. Hood
, E. Richard Moxon

University of Oxford
Centre For Ecology & Hydrology
Oxford University Hospitals NHS Foundation Trust
Maxwell Finland Lab. Infect. Dis.

Research output: Contribution to journal › Article › peer-review

58 Citations (SciVal)

Abstract

Non-typeable (NT) or capsule-deficient, Haemophilus influenzae (Hi) is a common commensal of the upper respiratory tract of humans and can be pathogenic resulting in diseases such as otitis media, sinusitis and pneumonia. The lipopolysaccharide (LPS) of NT Hi is a major virulence factor that displays substantial intra-strain and inter-strain variation of its oligosaccharide structures. To investigate the genetic basis of LPS variation we sequenced internal regions of each of seven genes required for the biosynthesis of either the inner or the outer core oligosaccharide structures. These sequences were obtained from 25 representative NT Hi isolates from episodes of otitis media. We found abundant evidence of recombination among LPS genes of NT Hi, a finding in marked contrast to previous analyses of biosynthetic genes for capsular polysaccharide, a well-documented virulence factor of Hi. We found mosaic sequences, linkage equilibrium between loci and a lack of congruence between gene trees. These high rates were not confined to LPS genes since evidence for similar amounts of recombination was also found in eight housekeeping genes in a subset of the same 25 isolates. These findings provide a population based foundation for a better understanding of the role of NT Hi LPS as a virulence factor and its potential as a candidate vaccine.

Original language	English
Pages (from-to)	57-66
Number of pages	10
Journal	Infection, Genetics and Evolution
Volume	3
Issue number	1
DOIs	https://doi.org/10.1016/S1567-1348(02)00152-1
Publication status	Published - 31 May 2003

Bibliographical note

Funding Information:
A.J.C. is funded by a research agreement with Aventis-Pasteur. D.F. was supported for part of this study as a Sloan/NSF Postdoctoral Fellowship in Molecular Evolution at the University of Oxford. M.E.D., D.W.H. and E.R.M. are funded by a Programme Grant from the Medical Research Council. R.G. and V.B. are funded by a research grant award to R.G. from the N.I.H. N.I.D.C.D. The authors wish to thank Juhani Eskola and the Finnish Otitis Media Study Group for the provision of strains used in this study.

Funding

A.J.C. is funded by a research agreement with Aventis-Pasteur. D.F. was supported for part of this study as a Sloan/NSF Postdoctoral Fellowship in Molecular Evolution at the University of Oxford. M.E.D., D.W.H. and E.R.M. are funded by a Programme Grant from the Medical Research Council. R.G. and V.B. are funded by a research grant award to R.G. from the N.I.H. N.I.D.C.D. The authors wish to thank Juhani Eskola and the Finnish Otitis Media Study Group for the provision of strains used in this study.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Bacterial population biology
Housekeeping genes
LPS biosynthetic genes
Non-typeable Haemophilus influenzae
Otitis media
Recombination

ASJC Scopus subject areas

Microbiology
Ecology, Evolution, Behavior and Systematics
Molecular Biology
Genetics
Microbiology (medical)
Infectious Diseases

Access to Document

10.1016/S1567-1348(02)00152-1

Cite this

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title = "High rates of recombination in otitis media isolates of non-typeable Haemophilus influenzae",

abstract = "Non-typeable (NT) or capsule-deficient, Haemophilus influenzae (Hi) is a common commensal of the upper respiratory tract of humans and can be pathogenic resulting in diseases such as otitis media, sinusitis and pneumonia. The lipopolysaccharide (LPS) of NT Hi is a major virulence factor that displays substantial intra-strain and inter-strain variation of its oligosaccharide structures. To investigate the genetic basis of LPS variation we sequenced internal regions of each of seven genes required for the biosynthesis of either the inner or the outer core oligosaccharide structures. These sequences were obtained from 25 representative NT Hi isolates from episodes of otitis media. We found abundant evidence of recombination among LPS genes of NT Hi, a finding in marked contrast to previous analyses of biosynthetic genes for capsular polysaccharide, a well-documented virulence factor of Hi. We found mosaic sequences, linkage equilibrium between loci and a lack of congruence between gene trees. These high rates were not confined to LPS genes since evidence for similar amounts of recombination was also found in eight housekeeping genes in a subset of the same 25 isolates. These findings provide a population based foundation for a better understanding of the role of NT Hi LPS as a virulence factor and its potential as a candidate vaccine.",

keywords = "Bacterial population biology, Housekeeping genes, LPS biosynthetic genes, Non-typeable Haemophilus influenzae, Otitis media, Recombination",

author = "Cody, \{Alison J.\} and Dawn Field and Feil, \{Edward J.\} and Suzanna Stringer and Deadman, \{Mary E.\} and Tsolaki, \{Anthony G.\} and Brett Gratz and Val{\'e}rie Bouchet and Richard Goldstein and Hood, \{Derek W.\} and Moxon, \{E. Richard\}",

note = "Funding Information: A.J.C. is funded by a research agreement with Aventis-Pasteur. D.F. was supported for part of this study as a Sloan/NSF Postdoctoral Fellowship in Molecular Evolution at the University of Oxford. M.E.D., D.W.H. and E.R.M. are funded by a Programme Grant from the Medical Research Council. R.G. and V.B. are funded by a research grant award to R.G. from the N.I.H. N.I.D.C.D. The authors wish to thank Juhani Eskola and the Finnish Otitis Media Study Group for the provision of strains used in this study. ",

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doi = "10.1016/S1567-1348(02)00152-1",

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T1 - High rates of recombination in otitis media isolates of non-typeable Haemophilus influenzae

AU - Cody, Alison J.

AU - Field, Dawn

AU - Feil, Edward J.

AU - Stringer, Suzanna

AU - Deadman, Mary E.

AU - Tsolaki, Anthony G.

AU - Gratz, Brett

AU - Bouchet, Valérie

AU - Goldstein, Richard

AU - Hood, Derek W.

AU - Moxon, E. Richard

N1 - Funding Information: A.J.C. is funded by a research agreement with Aventis-Pasteur. D.F. was supported for part of this study as a Sloan/NSF Postdoctoral Fellowship in Molecular Evolution at the University of Oxford. M.E.D., D.W.H. and E.R.M. are funded by a Programme Grant from the Medical Research Council. R.G. and V.B. are funded by a research grant award to R.G. from the N.I.H. N.I.D.C.D. The authors wish to thank Juhani Eskola and the Finnish Otitis Media Study Group for the provision of strains used in this study.

PY - 2003/5/31

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N2 - Non-typeable (NT) or capsule-deficient, Haemophilus influenzae (Hi) is a common commensal of the upper respiratory tract of humans and can be pathogenic resulting in diseases such as otitis media, sinusitis and pneumonia. The lipopolysaccharide (LPS) of NT Hi is a major virulence factor that displays substantial intra-strain and inter-strain variation of its oligosaccharide structures. To investigate the genetic basis of LPS variation we sequenced internal regions of each of seven genes required for the biosynthesis of either the inner or the outer core oligosaccharide structures. These sequences were obtained from 25 representative NT Hi isolates from episodes of otitis media. We found abundant evidence of recombination among LPS genes of NT Hi, a finding in marked contrast to previous analyses of biosynthetic genes for capsular polysaccharide, a well-documented virulence factor of Hi. We found mosaic sequences, linkage equilibrium between loci and a lack of congruence between gene trees. These high rates were not confined to LPS genes since evidence for similar amounts of recombination was also found in eight housekeeping genes in a subset of the same 25 isolates. These findings provide a population based foundation for a better understanding of the role of NT Hi LPS as a virulence factor and its potential as a candidate vaccine.

AB - Non-typeable (NT) or capsule-deficient, Haemophilus influenzae (Hi) is a common commensal of the upper respiratory tract of humans and can be pathogenic resulting in diseases such as otitis media, sinusitis and pneumonia. The lipopolysaccharide (LPS) of NT Hi is a major virulence factor that displays substantial intra-strain and inter-strain variation of its oligosaccharide structures. To investigate the genetic basis of LPS variation we sequenced internal regions of each of seven genes required for the biosynthesis of either the inner or the outer core oligosaccharide structures. These sequences were obtained from 25 representative NT Hi isolates from episodes of otitis media. We found abundant evidence of recombination among LPS genes of NT Hi, a finding in marked contrast to previous analyses of biosynthetic genes for capsular polysaccharide, a well-documented virulence factor of Hi. We found mosaic sequences, linkage equilibrium between loci and a lack of congruence between gene trees. These high rates were not confined to LPS genes since evidence for similar amounts of recombination was also found in eight housekeeping genes in a subset of the same 25 isolates. These findings provide a population based foundation for a better understanding of the role of NT Hi LPS as a virulence factor and its potential as a candidate vaccine.

KW - Bacterial population biology

KW - Housekeeping genes

KW - LPS biosynthetic genes

KW - Non-typeable Haemophilus influenzae

KW - Otitis media

KW - Recombination

UR - https://www.scopus.com/pages/publications/0037403514

U2 - 10.1016/S1567-1348(02)00152-1

DO - 10.1016/S1567-1348(02)00152-1

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AN - SCOPUS:0037403514

SN - 1567-1348

VL - 3

SP - 57

EP - 66

JO - Infection, Genetics and Evolution

JF - Infection, Genetics and Evolution

IS - 1

ER -

High rates of recombination in otitis media isolates of non-typeable Haemophilus influenzae

Abstract

Bibliographical note

Funding

UN SDGs

Keywords

ASJC Scopus subject areas

Access to Document

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