High plasma insulin-like growth factor-II and low lipid content in transgenic mice

measurements of lipid metabolism

T H Da Costa, D H Williamson, A Ward, P Bates, Rebecca Fisher, L Richardson, D J Hill, I C Robinson, C F Graham

Research output: Contribution to journalArticle

Abstract

Transgenic mice were made by introducing extra copies of the mouse insulin-like growth factor-II (IGF-II) gene driven by the bovine keratin 10 promoter (BKVI). The adult plasma IGF-II levels were elevated at least three times in one line. In this line, there was a lower lipid content of both brown and white adipose depots at 2-4 months of age, and 40% less fat in the carcass at 7-9 months. The low lipid phenotype was not detected in the carcass at 2 weeks after birth. The lean characteristic was attributed to circulating IGF-II because the transgene was not expressed in fat. At 2-4 months of age, the transgenes oxidized more oral lipid, and less of this lipid was incorporated into the whole body and the epididymal fat. In contrast, the interscapular brown adipose tissue maintained lipid incorporation and lipoprotein lipase activity despite its reduced size. The altered activity of the brown adipose tissue may account for the gradual onset and persistence of the lean feature of the transgenic mice. There were no substantial changes in lipogenesis which could account for the low fat content. The plasma levels of IGF-I, insulin, glycerol, non-esterified fatty acids, triacylglycerols and glucose were not greatly changed and the pituitary GH content was within the normal range.

Original languageEnglish
Pages (from-to)433-9
Number of pages7
JournalJournal of Endocrinology
Volume143
Issue number3
Publication statusPublished - Dec 1994

Fingerprint

Insulin-Like Growth Factor II
Lipid Metabolism
Transgenic Mice
Lipids
Fats
Brown Adipose Tissue
Transgenes
Keratin-10
Lipogenesis
Lipoprotein Lipase
Insulin-Like Growth Factor I
Reference Values
Triglycerides
Fatty Acids
Parturition
Phenotype
Glucose
Genes

Keywords

  • Adipose Tissue
  • Animals
  • Dietary Fats
  • Insulin-Like Growth Factor II
  • Lipids
  • Lipoprotein Lipase
  • Mice
  • Mice, Transgenic
  • Journal Article

Cite this

High plasma insulin-like growth factor-II and low lipid content in transgenic mice : measurements of lipid metabolism. / Da Costa, T H; Williamson, D H; Ward, A; Bates, P; Fisher, Rebecca; Richardson, L; Hill, D J; Robinson, I C; Graham, C F.

In: Journal of Endocrinology, Vol. 143, No. 3, 12.1994, p. 433-9.

Research output: Contribution to journalArticle

Da Costa, TH, Williamson, DH, Ward, A, Bates, P, Fisher, R, Richardson, L, Hill, DJ, Robinson, IC & Graham, CF 1994, 'High plasma insulin-like growth factor-II and low lipid content in transgenic mice: measurements of lipid metabolism', Journal of Endocrinology, vol. 143, no. 3, pp. 433-9.
Da Costa, T H ; Williamson, D H ; Ward, A ; Bates, P ; Fisher, Rebecca ; Richardson, L ; Hill, D J ; Robinson, I C ; Graham, C F. / High plasma insulin-like growth factor-II and low lipid content in transgenic mice : measurements of lipid metabolism. In: Journal of Endocrinology. 1994 ; Vol. 143, No. 3. pp. 433-9.
@article{044a3c16e61047a7a4c9f04c64c9def1,
title = "High plasma insulin-like growth factor-II and low lipid content in transgenic mice: measurements of lipid metabolism",
abstract = "Transgenic mice were made by introducing extra copies of the mouse insulin-like growth factor-II (IGF-II) gene driven by the bovine keratin 10 promoter (BKVI). The adult plasma IGF-II levels were elevated at least three times in one line. In this line, there was a lower lipid content of both brown and white adipose depots at 2-4 months of age, and 40{\%} less fat in the carcass at 7-9 months. The low lipid phenotype was not detected in the carcass at 2 weeks after birth. The lean characteristic was attributed to circulating IGF-II because the transgene was not expressed in fat. At 2-4 months of age, the transgenes oxidized more oral lipid, and less of this lipid was incorporated into the whole body and the epididymal fat. In contrast, the interscapular brown adipose tissue maintained lipid incorporation and lipoprotein lipase activity despite its reduced size. The altered activity of the brown adipose tissue may account for the gradual onset and persistence of the lean feature of the transgenic mice. There were no substantial changes in lipogenesis which could account for the low fat content. The plasma levels of IGF-I, insulin, glycerol, non-esterified fatty acids, triacylglycerols and glucose were not greatly changed and the pituitary GH content was within the normal range.",
keywords = "Adipose Tissue, Animals, Dietary Fats, Insulin-Like Growth Factor II, Lipids, Lipoprotein Lipase, Mice, Mice, Transgenic, Journal Article",
author = "{Da Costa}, {T H} and Williamson, {D H} and A Ward and P Bates and Rebecca Fisher and L Richardson and Hill, {D J} and Robinson, {I C} and Graham, {C F}",
year = "1994",
month = "12",
language = "English",
volume = "143",
pages = "433--9",
journal = "Journal of Endocrinology",
issn = "0022-0795",
publisher = "Society for Endocrinology",
number = "3",

}

TY - JOUR

T1 - High plasma insulin-like growth factor-II and low lipid content in transgenic mice

T2 - measurements of lipid metabolism

AU - Da Costa, T H

AU - Williamson, D H

AU - Ward, A

AU - Bates, P

AU - Fisher, Rebecca

AU - Richardson, L

AU - Hill, D J

AU - Robinson, I C

AU - Graham, C F

PY - 1994/12

Y1 - 1994/12

N2 - Transgenic mice were made by introducing extra copies of the mouse insulin-like growth factor-II (IGF-II) gene driven by the bovine keratin 10 promoter (BKVI). The adult plasma IGF-II levels were elevated at least three times in one line. In this line, there was a lower lipid content of both brown and white adipose depots at 2-4 months of age, and 40% less fat in the carcass at 7-9 months. The low lipid phenotype was not detected in the carcass at 2 weeks after birth. The lean characteristic was attributed to circulating IGF-II because the transgene was not expressed in fat. At 2-4 months of age, the transgenes oxidized more oral lipid, and less of this lipid was incorporated into the whole body and the epididymal fat. In contrast, the interscapular brown adipose tissue maintained lipid incorporation and lipoprotein lipase activity despite its reduced size. The altered activity of the brown adipose tissue may account for the gradual onset and persistence of the lean feature of the transgenic mice. There were no substantial changes in lipogenesis which could account for the low fat content. The plasma levels of IGF-I, insulin, glycerol, non-esterified fatty acids, triacylglycerols and glucose were not greatly changed and the pituitary GH content was within the normal range.

AB - Transgenic mice were made by introducing extra copies of the mouse insulin-like growth factor-II (IGF-II) gene driven by the bovine keratin 10 promoter (BKVI). The adult plasma IGF-II levels were elevated at least three times in one line. In this line, there was a lower lipid content of both brown and white adipose depots at 2-4 months of age, and 40% less fat in the carcass at 7-9 months. The low lipid phenotype was not detected in the carcass at 2 weeks after birth. The lean characteristic was attributed to circulating IGF-II because the transgene was not expressed in fat. At 2-4 months of age, the transgenes oxidized more oral lipid, and less of this lipid was incorporated into the whole body and the epididymal fat. In contrast, the interscapular brown adipose tissue maintained lipid incorporation and lipoprotein lipase activity despite its reduced size. The altered activity of the brown adipose tissue may account for the gradual onset and persistence of the lean feature of the transgenic mice. There were no substantial changes in lipogenesis which could account for the low fat content. The plasma levels of IGF-I, insulin, glycerol, non-esterified fatty acids, triacylglycerols and glucose were not greatly changed and the pituitary GH content was within the normal range.

KW - Adipose Tissue

KW - Animals

KW - Dietary Fats

KW - Insulin-Like Growth Factor II

KW - Lipids

KW - Lipoprotein Lipase

KW - Mice

KW - Mice, Transgenic

KW - Journal Article

M3 - Article

VL - 143

SP - 433

EP - 439

JO - Journal of Endocrinology

JF - Journal of Endocrinology

SN - 0022-0795

IS - 3

ER -