High-intensity training reduces CD8+ T-cell redistribution in response to exercise

Oliver C Witard, James E Turner, Sarah R Jackman, Kevin D Tipton, Asker E Jeukendrup, Arie K Kies, Jos A Bosch

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24 Citations (Scopus)
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Abstract

Purpose: We examined whether exercise-induced lymphocytosis and lymphocytopenia are impaired with high-intensity training. Methods: Eight trained cyclists (V̇O2max = 64.2 ± 6.5 mL•kg -1•min-1) undertook 1 wk of normal-intensity training and a second week of high-intensity training. On day 7 of each week, participants performed a cycling task, consisting of 120 min of submaximal exercise followed by a 45-min time trial. Blood was collected before, during, and after exercise. CD8+ T lymphocytes (CD8TLs) were identified, as well as CD8TL subpopulations on the basis of CD45RA and CD27 expression. Results: High-intensity training (18,577 ± 10,984 cells per microliter × ∼165 min) was associated with a smaller exercise-induced mobilization of CD8+TLs compared with normal-intensity training (28,473 ± 16,163 cells per microliter × ∼165 min, P = 0.09). The response of highly cytotoxic CD8+TLs (CD45RA+CD27 -) to exercise was smaller after 1 wk of high-intensity training (3144 ± 924 cells per microliter × ∼165 min) compared with normal-intensity training (6417 ± 2143 cells per microliter × ∼165 min, P < 0.05). High-intensity training reduced postexercise CD8+TL lymphocytopenia (-436 ± 234 cells per microliter) compared with normal-intensity training (-630 ± 320 cells per microliter, P < 0.05). This was driven by a reduced egress of naive CD8+TLs (CD27+CD45RA+). High-intensity training was associated with reduced plasma epinephrine (-37%) and cortisol (-15%) responses (P < 0.05). Conclusions: High-intensity training impaired CD8+TL mobilization and egress in response to exercise. Highly cytotoxic CD8 +TLs were primarily responsible for the reduced mobilization of CD8+TLs, which occurred in parallel with smaller neuroendocrine responses. The reduced capacity for CD8+TLs to leave blood after exercise with high-intensity training was accounted for primarily by naive, and also, highly cytotoxic CD8+TLs. This impaired CD8+TL redistribution in athletes undertaking intensified training may imply reduced immune surveillance.
Original languageEnglish
Pages (from-to)1689-1697
Number of pages9
JournalMedicine & Science in Sports & Exercise
Volume44
Issue number9
DOIs
Publication statusPublished - Sep 2012

Keywords

  • Adult
  • CD8-Positive T-Lymphocytes
  • Exercise
  • Flow Cytometry
  • Humans
  • Lymphocytosis
  • Lymphopenia
  • Male
  • Oxygen Consumption

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