TY - JOUR
T1 - High fidelity fibre-based physiological sensing deep in tissue
AU - Choudhary, Tushar R.
AU - Tanner, Michael G.
AU - Megia-Fernandez, Alicia
AU - Harrington, Kerrianne
AU - Wood, Harry A.
AU - Marshall, Adam
AU - Zhu, Patricia
AU - Chankeshwara, Sunay V.
AU - Choudhury, Debaditya
AU - Monro, Graham
AU - Ucuncu, Muhammed
AU - Yu, Fei
AU - Duncan, Rory R.
AU - Thomson, Robert R.
AU - Dhaliwal, Kevin
AU - Bradley, Mark
PY - 2019/5/22
Y1 - 2019/5/22
N2 - Physiological sensing deep in tissue remains a clinical challenge. Here a flexible miniaturised sensing optrode providing a platform to perform minimally invasive in vivo in situ measurements is reported. Silica microspheres covalently coupled with a high density of ratiometrically configured fluorophores were deposited into etched pits on the distal end of a 150 µm diameter multicore optical fibre. With this platform, photonic measurements of pH and oxygen concentration with high precision in the distal alveolar space of the lung are reported. We demonstrated the phenomenon that high-density deposition of carboxyfluorescein covalently coupled to silica microspheres shows an inverse shift in fluorescence in response to varying pH. This platform delivered fast and accurate measurements (±0.02 pH units and ±0.6 mg/L of oxygen), near instantaneous response time and a flexible architecture for addition of multiple sensors.
AB - Physiological sensing deep in tissue remains a clinical challenge. Here a flexible miniaturised sensing optrode providing a platform to perform minimally invasive in vivo in situ measurements is reported. Silica microspheres covalently coupled with a high density of ratiometrically configured fluorophores were deposited into etched pits on the distal end of a 150 µm diameter multicore optical fibre. With this platform, photonic measurements of pH and oxygen concentration with high precision in the distal alveolar space of the lung are reported. We demonstrated the phenomenon that high-density deposition of carboxyfluorescein covalently coupled to silica microspheres shows an inverse shift in fluorescence in response to varying pH. This platform delivered fast and accurate measurements (±0.02 pH units and ±0.6 mg/L of oxygen), near instantaneous response time and a flexible architecture for addition of multiple sensors.
UR - http://www.scopus.com/inward/record.url?scp=85066149386&partnerID=8YFLogxK
U2 - 10.1038/s41598-019-44077-7
DO - 10.1038/s41598-019-44077-7
M3 - Article
C2 - 31118459
AN - SCOPUS:85066149386
SN - 2045-2322
VL - 9
SP - 1
EP - 10
JO - Scientific Reports
JF - Scientific Reports
IS - 1
M1 - 7713
ER -