Heterologous Production of Functional Forms of Rhizopus oryzae Lipase in Escherichia coli

Mirella Di Lorenzo; A Hidalgo; M Haas; U T Bornscheuer

doi:10.1128/AEM.71.12.8974-8977.2005

Heterologous Production of Functional Forms of Rhizopus oryzae Lipase in Escherichia coli

Mirella Di Lorenzo, A Hidalgo, M Haas, U T Bornscheuer

Department of Chemical Engineering

Research output: Contribution to journal › Article › peer-review

37 Citations (SciVal)

Abstract

To date, expression of the lipase from Rhizopus oryzae (ROL) in Escherichia coli always led to the formation of inclusion bodies and inactive protein. However, the production of active ROL and its precursor ProROL in soluble form was achieved when E. coli Origami(DE3) and pET-11d were used as expression systems.

Original language	English
Pages (from-to)	8974-8977
Number of pages	4
Journal	Applied and Environmental Microbiology
Volume	71
Issue number	12
DOIs	https://doi.org/10.1128/AEM.71.12.8974-8977.2005
Publication status	Published - 1 Dec 2005

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10.1128/AEM.71.12.8974-8977.2005

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title = "Heterologous Production of Functional Forms of Rhizopus oryzae Lipase in Escherichia coli",

abstract = "To date, expression of the lipase from Rhizopus oryzae (ROL) in Escherichia coli always led to the formation of inclusion bodies and inactive protein. However, the production of active ROL and its precursor ProROL in soluble form was achieved when E. coli Origami(DE3) and pET-11d were used as expression systems.",

author = "{Di Lorenzo}, Mirella and A Hidalgo and M Haas and Bornscheuer, {U T}",

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AU - Di Lorenzo, Mirella

AU - Hidalgo, A

AU - Haas, M

AU - Bornscheuer, U T

PY - 2005/12/1

Y1 - 2005/12/1

N2 - To date, expression of the lipase from Rhizopus oryzae (ROL) in Escherichia coli always led to the formation of inclusion bodies and inactive protein. However, the production of active ROL and its precursor ProROL in soluble form was achieved when E. coli Origami(DE3) and pET-11d were used as expression systems.

AB - To date, expression of the lipase from Rhizopus oryzae (ROL) in Escherichia coli always led to the formation of inclusion bodies and inactive protein. However, the production of active ROL and its precursor ProROL in soluble form was achieved when E. coli Origami(DE3) and pET-11d were used as expression systems.

U2 - 10.1128/AEM.71.12.8974-8977.2005

DO - 10.1128/AEM.71.12.8974-8977.2005

M3 - Article

SN - 0099-2240

VL - 71

SP - 8974

EP - 8977

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 12

ER -

Heterologous Production of Functional Forms of Rhizopus oryzae Lipase in Escherichia coli

Abstract

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