Heterologous Production of Functional Forms of Rhizopus oryzae Lipase in Escherichia coli

Mirella Di Lorenzo, A Hidalgo, M Haas, U T Bornscheuer

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

To date, expression of the lipase from Rhizopus oryzae (ROL) in Escherichia coli always led to the formation of inclusion bodies and inactive protein. However, the production of active ROL and its precursor ProROL in soluble form was achieved when E. coli Origami(DE3) and pET-11d were used as expression systems.
Original languageEnglish
Pages (from-to)8974-8977
Number of pages4
JournalApplied and Environmental Microbiology
Volume71
Issue number12
DOIs
Publication statusPublished - Dec 2005

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