Heat shock factor 1 is a substrate for p38 mitogen-activated Protein Kinases

Sharadha Dayalan Naidu, Calum Sutherland, Ying Zhang, Ana Risco, Laureano de la Vega, Christopher J Caunt, C James Hastie, Douglas J Lamont, Laura Torrente, Sudhir Chowdhry, Ivor J Benjamin, Stephen M Keyse, Ana Cuenda, Albena T Dinkova-Kostova

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Abstract

Heat Shock Factor 1 (HSF1) monitors the structural integrity of the proteome. Phosphorylation at S326 is a hallmark for HSF1 activation, but the identity of the kinase(s) phosphorylating this site has remained elusive. We show that the dietary agent phenethyl isothiocyanate (PEITC) inhibits heat shock protein 90 (Hsp90), the main negative regulator of HSF1, activates p38 MAPK, increases S326 phosphorylation, trimerization and nuclear translocation of HSF1, and the transcription of a luciferase reporter as well as the endogenous prototypic HSF1 target Hsp70. In vitro, all members of the p38 mitogen-activated protein kinase (MAPK) family rapidly and stoichiometrically catalyze the S326 phosphorylation. The use of stable knockdown cell lines and inhibitors indicated that among the p38 MAPK, p38γ is the principal isoform responsible for the phosphorylation of HSF1 at S326 in cells. A protease-mass spectrometry approach confirmed S326 phosphorylation, and unexpectedly, revealed that p38 MAPK also catalyze phosphorylation of HSF1 at S303/307, previously known repressive post-translational modifications. Thus, we have identified p38 MAPK as highly efficient catalysts for the phosphorylation of HSF1. Furthermore, our findings suggest that the magnitude and persistence of activation of p38 MAPK are important determinants of the extent and duration of the heat shock response.

LanguageEnglish
Pages2403-2417
JournalMolecular and Cellular Biology
Volume36
Issue number18
DOIs
StatusPublished - Sep 2016

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p38 Mitogen-Activated Protein Kinases
Shock
Hot Temperature
Phosphorylation
HSP90 Heat-Shock Proteins
Heat-Shock Response
Proteome
Post Translational Protein Processing
Luciferases
Mass Spectrometry
Protein Isoforms
Peptide Hydrolases
Phosphotransferases
Cell Line

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Dayalan Naidu, S., Sutherland, C., Zhang, Y., Risco, A., de la Vega, L., Caunt, C. J., ... Dinkova-Kostova, A. T. (2016). Heat shock factor 1 is a substrate for p38 mitogen-activated Protein Kinases. DOI: 10.1128/MCB.00292-16

Heat shock factor 1 is a substrate for p38 mitogen-activated Protein Kinases. / Dayalan Naidu, Sharadha; Sutherland, Calum; Zhang, Ying; Risco, Ana; de la Vega, Laureano; Caunt, Christopher J; Hastie, C James; Lamont, Douglas J; Torrente, Laura; Chowdhry, Sudhir; Benjamin, Ivor J; Keyse, Stephen M; Cuenda, Ana; Dinkova-Kostova, Albena T.

In: Molecular and Cellular Biology, Vol. 36, No. 18, 09.2016, p. 2403-2417.

Research output: Contribution to journalArticle

Dayalan Naidu, S, Sutherland, C, Zhang, Y, Risco, A, de la Vega, L, Caunt, CJ, Hastie, CJ, Lamont, DJ, Torrente, L, Chowdhry, S, Benjamin, IJ, Keyse, SM, Cuenda, A & Dinkova-Kostova, AT 2016, 'Heat shock factor 1 is a substrate for p38 mitogen-activated Protein Kinases' Molecular and Cellular Biology, vol. 36, no. 18, pp. 2403-2417. DOI: 10.1128/MCB.00292-16
Dayalan Naidu S, Sutherland C, Zhang Y, Risco A, de la Vega L, Caunt CJ et al. Heat shock factor 1 is a substrate for p38 mitogen-activated Protein Kinases. Molecular and Cellular Biology. 2016 Sep;36(18):2403-2417. Available from, DOI: 10.1128/MCB.00292-16
Dayalan Naidu, Sharadha ; Sutherland, Calum ; Zhang, Ying ; Risco, Ana ; de la Vega, Laureano ; Caunt, Christopher J ; Hastie, C James ; Lamont, Douglas J ; Torrente, Laura ; Chowdhry, Sudhir ; Benjamin, Ivor J ; Keyse, Stephen M ; Cuenda, Ana ; Dinkova-Kostova, Albena T. / Heat shock factor 1 is a substrate for p38 mitogen-activated Protein Kinases. In: Molecular and Cellular Biology. 2016 ; Vol. 36, No. 18. pp. 2403-2417
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abstract = "Heat Shock Factor 1 (HSF1) monitors the structural integrity of the proteome. Phosphorylation at S326 is a hallmark for HSF1 activation, but the identity of the kinase(s) phosphorylating this site has remained elusive. We show that the dietary agent phenethyl isothiocyanate (PEITC) inhibits heat shock protein 90 (Hsp90), the main negative regulator of HSF1, activates p38 MAPK, increases S326 phosphorylation, trimerization and nuclear translocation of HSF1, and the transcription of a luciferase reporter as well as the endogenous prototypic HSF1 target Hsp70. In vitro, all members of the p38 mitogen-activated protein kinase (MAPK) family rapidly and stoichiometrically catalyze the S326 phosphorylation. The use of stable knockdown cell lines and inhibitors indicated that among the p38 MAPK, p38γ is the principal isoform responsible for the phosphorylation of HSF1 at S326 in cells. A protease-mass spectrometry approach confirmed S326 phosphorylation, and unexpectedly, revealed that p38 MAPK also catalyze phosphorylation of HSF1 at S303/307, previously known repressive post-translational modifications. Thus, we have identified p38 MAPK as highly efficient catalysts for the phosphorylation of HSF1. Furthermore, our findings suggest that the magnitude and persistence of activation of p38 MAPK are important determinants of the extent and duration of the heat shock response.",
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AU - Dayalan Naidu,Sharadha

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AU - Zhang,Ying

AU - Risco,Ana

AU - de la Vega,Laureano

AU - Caunt,Christopher J

AU - Hastie,C James

AU - Lamont,Douglas J

AU - Torrente,Laura

AU - Chowdhry,Sudhir

AU - Benjamin,Ivor J

AU - Keyse,Stephen M

AU - Cuenda,Ana

AU - Dinkova-Kostova,Albena T

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N2 - Heat Shock Factor 1 (HSF1) monitors the structural integrity of the proteome. Phosphorylation at S326 is a hallmark for HSF1 activation, but the identity of the kinase(s) phosphorylating this site has remained elusive. We show that the dietary agent phenethyl isothiocyanate (PEITC) inhibits heat shock protein 90 (Hsp90), the main negative regulator of HSF1, activates p38 MAPK, increases S326 phosphorylation, trimerization and nuclear translocation of HSF1, and the transcription of a luciferase reporter as well as the endogenous prototypic HSF1 target Hsp70. In vitro, all members of the p38 mitogen-activated protein kinase (MAPK) family rapidly and stoichiometrically catalyze the S326 phosphorylation. The use of stable knockdown cell lines and inhibitors indicated that among the p38 MAPK, p38γ is the principal isoform responsible for the phosphorylation of HSF1 at S326 in cells. A protease-mass spectrometry approach confirmed S326 phosphorylation, and unexpectedly, revealed that p38 MAPK also catalyze phosphorylation of HSF1 at S303/307, previously known repressive post-translational modifications. Thus, we have identified p38 MAPK as highly efficient catalysts for the phosphorylation of HSF1. Furthermore, our findings suggest that the magnitude and persistence of activation of p38 MAPK are important determinants of the extent and duration of the heat shock response.

AB - Heat Shock Factor 1 (HSF1) monitors the structural integrity of the proteome. Phosphorylation at S326 is a hallmark for HSF1 activation, but the identity of the kinase(s) phosphorylating this site has remained elusive. We show that the dietary agent phenethyl isothiocyanate (PEITC) inhibits heat shock protein 90 (Hsp90), the main negative regulator of HSF1, activates p38 MAPK, increases S326 phosphorylation, trimerization and nuclear translocation of HSF1, and the transcription of a luciferase reporter as well as the endogenous prototypic HSF1 target Hsp70. In vitro, all members of the p38 mitogen-activated protein kinase (MAPK) family rapidly and stoichiometrically catalyze the S326 phosphorylation. The use of stable knockdown cell lines and inhibitors indicated that among the p38 MAPK, p38γ is the principal isoform responsible for the phosphorylation of HSF1 at S326 in cells. A protease-mass spectrometry approach confirmed S326 phosphorylation, and unexpectedly, revealed that p38 MAPK also catalyze phosphorylation of HSF1 at S303/307, previously known repressive post-translational modifications. Thus, we have identified p38 MAPK as highly efficient catalysts for the phosphorylation of HSF1. Furthermore, our findings suggest that the magnitude and persistence of activation of p38 MAPK are important determinants of the extent and duration of the heat shock response.

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