TY - JOUR
T1 - Gold nanoparticle decorated pH-sensitive polymeric nanocontainers as a potential theranostic agent
AU - Theodosiou, M.
AU - Boukos, N.
AU - Sakellis, E.
AU - Zachariadis, Michael
AU - Efthimiadou, Eleni K.
N1 - Funding Information:
The research work was supported by the Hellenic Foundation for Research and Innovation (HFRI) and the General Secretariat for Research and Technology (GSRT) , under the HFRI PhD Fellowship grant (GA. no. 14650 ).
Publisher Copyright:
© 2019 Elsevier B.V.
PY - 2019/11/1
Y1 - 2019/11/1
N2 - A pH-sensitive system of hollow P(MAA-co-MBA-co-AA) nanocontainers (NCs) modified with gold nanoparticles (GNCs) has been developed for theranostic applications, drug delivery and real time monitoring through imaging. The GNCs were synthesised by the distillation precipitation copolymerization procedure followed by in situ synthesis and embodiment of gold nanoparticles on the polymeric matrix (CSNs). Separately, citrate capped gold nanoparticles (GNPs) were also synthesized and compared with the GNCs for their fluorescence and cellular localization ability. The GNCs were tested for their drug loading and release behavior in response to the anticancer drug doxorubicin (DOX) at different pH values. Sustained drug release was observed at acidic pH. The viability of MCF-7 breast cancer cells and HEK-293 human embryonic kidney cells in relation to the GNCs, GNPs, GNC@DOX and DOX was also evaluated. GNCs and GNPs at the tested concentrations did not inhibit proliferation at either cell lines, whereas the GNCs@DOX presented comparable results. Cellular migration of MCF-7 cells treated with GNCs or GNPs was evaluated through the scratch-wound healing assay but no significant inhibition was detected. GNCs’ and GNPs’ fluorescence ability was exploited for assessing cellular localization through confocal laser scanning microscopy. GNCs after only 1 h of treatment were found in the cytoplasm of MCF7 cells, whereas GNCs@DOX were localized in the nucleus; the desirable site of action of DOX.
AB - A pH-sensitive system of hollow P(MAA-co-MBA-co-AA) nanocontainers (NCs) modified with gold nanoparticles (GNCs) has been developed for theranostic applications, drug delivery and real time monitoring through imaging. The GNCs were synthesised by the distillation precipitation copolymerization procedure followed by in situ synthesis and embodiment of gold nanoparticles on the polymeric matrix (CSNs). Separately, citrate capped gold nanoparticles (GNPs) were also synthesized and compared with the GNCs for their fluorescence and cellular localization ability. The GNCs were tested for their drug loading and release behavior in response to the anticancer drug doxorubicin (DOX) at different pH values. Sustained drug release was observed at acidic pH. The viability of MCF-7 breast cancer cells and HEK-293 human embryonic kidney cells in relation to the GNCs, GNPs, GNC@DOX and DOX was also evaluated. GNCs and GNPs at the tested concentrations did not inhibit proliferation at either cell lines, whereas the GNCs@DOX presented comparable results. Cellular migration of MCF-7 cells treated with GNCs or GNPs was evaluated through the scratch-wound healing assay but no significant inhibition was detected. GNCs’ and GNPs’ fluorescence ability was exploited for assessing cellular localization through confocal laser scanning microscopy. GNCs after only 1 h of treatment were found in the cytoplasm of MCF7 cells, whereas GNCs@DOX were localized in the nucleus; the desirable site of action of DOX.
KW - Cancer theranostics
KW - Drug delivery system
KW - Gold nanoparticles
KW - Imaging
KW - Nanomedicine
KW - Polymeric nanocontainers
UR - http://www.scopus.com/inward/record.url?scp=85070607903&partnerID=8YFLogxK
U2 - 10.1016/j.colsurfb.2019.110420
DO - 10.1016/j.colsurfb.2019.110420
M3 - Article
C2 - 31421401
AN - SCOPUS:85070607903
VL - 183
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
SN - 0927-7765
M1 - 110420
ER -
